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Western Blot (WB) (Western Blot Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, L02 whole cell lysate, HepF2 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate All lanes: TOMM22 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16 kDa Observed band size: 18 kDa)

TOMM22 recombinant antibody

TOMM22 Antibody

Gene Names
TOMM22; 1C9-2; TOM22; MST065; MSTP065
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity-chromatography
Synonyms
TOMM22; Monoclonal Recombinant Antibody; TOMM22 Antibody; Mitochondrial import receptor subunit TOM22 homolog (hTom22) (1C9-2) (Translocase of outer membrane 22 kDa subunit homolog); TOM22; TOMM22 recombinant antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
7C11
Purity/Purification
Affinity-chromatography
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, PH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for TOMM22 recombinant antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:5000
IHC: 1:50-1:200
Antibody Type
Recombinant Antibody
Conjugation
Non-conjugated
Immunogen
A synthesized peptide derived from human TOMM22
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western Blot Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, L02 whole cell lysate, HepF2 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate All lanes: TOMM22 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16 kDa Observed band size: 18 kDa)

Western Blot (WB) (Western Blot Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, L02 whole cell lysate, HepF2 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate All lanes: TOMM22 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16 kDa Observed band size: 18 kDa)

Immunohistochemistry (IHC)

(IHC image diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)

Immunohistochemistry (IHC) (IHC image diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
Related Product Information for TOMM22 recombinant antibody
Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore.
Product Categories/Family for TOMM22 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
142
NCBI Official Full Name
Mitochondrial import receptor subunit TOM22 homolog
NCBI Official Synonym Full Names
translocase of outer mitochondrial membrane 22 homolog (yeast)
NCBI Official Symbol
TOMM22
NCBI Official Synonym Symbols
1C9-2; TOM22; MST065; MSTP065
NCBI Protein Information
mitochondrial import receptor subunit TOM22 homolog; mitochondrial import receptor Tom22; translocase of outer membrane 22 kDa subunit homolog
UniProt Protein Name
Mitochondrial import receptor subunit TOM22 homolog
UniProt Gene Name
TOMM22
UniProt Synonym Gene Names
TOM22; hTom22
UniProt Entry Name
TOM22_HUMAN

NCBI Description

The protein encoded by this gene is an integral membrane protein of the mitochondrial outer membrane. The encoded protein interacts with TOMM20 and TOMM40, and forms a complex with several other proteins to import cytosolic preproteins into the mitochondrion. [provided by RefSeq, Jul 2008]

Uniprot Description

TOMM22: Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore. Belongs to the Tom22 family.

Protein type: Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: mitochondrion; membrane; mitochondrial outer membrane translocase complex; mitochondrial inner membrane; integral to membrane

Molecular Function: protein binding; protein transmembrane transporter activity

Biological Process: cellular protein metabolic process; protein targeting to mitochondrion; protein import into mitochondrial outer membrane

Research Articles on TOMM22

Similar Products

Product Notes

The TOMM22 tomm22 (Catalog #AAA7136621) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TOMM22 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TOMM22 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:5000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the TOMM22 tomm22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TOMM22, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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