Rabbit anti-Mouse TNFa  Monoclonal Antibody | anti-TNFa  antibody

Rabbit anti Mouse TNFa  Monoclonal Antibody

Cat. Num. AAA2544557

• Gene Names: TNF; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: TNFa ; Monoclonal Antibody; Rabbit anti Mouse TNFa Monoclonal Antibody; anti-TNFa antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 233

• Applicable Applications for anti-TNFa antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Mouse TNFa. The detection limit for Mouse TNFa is approximately 0.078 ng/well.
• Immunogen: Recombinant Mouse TNF-alpha/TNFa protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -70 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-TNFa  antibody

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

NCBI and Uniprot Product Information

• NCBI GI #: 37212
• NCBI GeneID: 7124
• UniProt Accession #: P01375; O43647; Q9P1Q2; Q9UIV3
• Molecular Weight: 25,644 Da
• NCBI Official Full Name: tumor necrosis factor, Tnfa
• NCBI Official Synonym Full Names: tumor necrosis factor
• NCBI Official Symbol: TNF
• NCBI Official Synonym Symbols: DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
• NCBI Protein Information: tumor necrosis factor
• UniProt Protein Name: Tumor necrosis factor
• UniProt Gene Name: TNF
• UniProt Synonym Gene Names: TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
• UniProt Entry Name: TNFA_HUMAN

NCBI Description

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

Uniprot Description

TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family.

Protein type: Apoptosis; Cytokine; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell surface; external side of plasma membrane; extracellular region; extracellular space; integral to plasma membrane; lipid raft; phagocytic cup; plasma membrane; recycling endosome

Molecular Function: cytokine activity; identical protein binding; protease binding; protein binding; tumor necrosis factor receptor binding

Biological Process: activation of MAPK activity; activation of MAPKKK activity; activation of NF-kappaB transcription factor; caspase activation; chronic inflammatory response to antigenic stimulus; cortical actin cytoskeleton organization and biogenesis; embryonic gut development; I-kappaB kinase/NF-kappaB cascade; induction of apoptosis via death domain receptors; inflammatory response; leukocyte tethering or rolling; lipopolysaccharide-mediated signaling pathway; MAPKKK cascade; negative regulation of cytokine secretion during immune response; negative regulation of interleukin-6 production; negative regulation of lipid catabolic process; negative regulation of protein complex disassembly; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of viral genome replication; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of cell adhesion; positive regulation of chemokine biosynthetic process; positive regulation of chemokine production; positive regulation of cytokine production; positive regulation of cytokine secretion; positive regulation of fever; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 production; positive regulation of JNK activity; positive regulation of MAP kinase activity; positive regulation of membrane protein ectodomain proteolysis; positive regulation of NF-kappaB import into nucleus; positive regulation of NFAT protein import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of osteoclast differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of phagocytosis; positive regulation of programmed cell death; positive regulation of protein amino acid phosphorylation; positive regulation of protein complex assembly; positive regulation of protein complex disassembly; positive regulation of protein kinase activity; positive regulation of protein transport; positive regulation of smooth muscle cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein import into nucleus, translocation; protein kinase B signaling cascade; receptor biosynthetic process; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of insulin secretion; response to glucocorticoid stimulus; response to salt stress; response to virus; sequestering of triacylglycerol; tumor necrosis factor-mediated signaling pathway

Disease: Asthma, Susceptibility To; Malaria, Susceptibility To; Migraine With Or Without Aura, Susceptibility To, 1