Mouse TNF-RI Monoclonal Antibody | anti-TNF-RI antibody

TNF-RI, Human, mAb H398, FITC

Cat. Num. AAA584218

• Reactivity: Reactivity: Human. Cross reactivity: Rat
• Applications: Western Blot
• Synonyms: TNF-RI; Monoclonal Antibody; Human; mAb H398; FITC; CD120a; Tumor necrosis factor receptor superfamily member 1A; p55/p60; TNFR-1; anti-TNF-RI antibody

• Host: Mouse
• Reactivity: Reactivity: Human. Cross reactivity: Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02 % sodium azide.
• Sequence Length: 471

• Applicable Applications for anti-TNF-RI antibody: Western Blot (WB)
• Application Notes: Application Use: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Conjugate: FITC
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-TNF-RI antibody

Product Description: The monoclonal antibody H398 recognizes the extracellular part of the Tumor Necrosis Factor Receptor type I (TNF-RI) of the membrane-bound as well as the soluble receptor. TNF-RI (~55-60 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha. TNF-RI signaling is initiated when trimeric TNF-alpha binds TNF-RI receptors. Subsequent TNF-RI trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD), Receptor Interacting Protein (RIP), cellular Inhibitor of Apoptosis Protein 1 (cIAP1), TNF Receptor Associated Factor 2 (TRAF2), and likely TRAF5. Studies with TNF-RI-deficient mice indicate that TNF-RI mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways.

References

1. Thoma, B et al; Identification of a 60 kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses. J Exp Med 1990, 172: 1019 2. Grell, M et al; TR60 and TR80 tumor necrosis factor (TNF)-receptors can independently mediate cytolysis. Lymphokine Cytokine Res 1993, 12: 143
3. Scheurich, P et al; Agonistic and antagonistic antibodies as a tool to study the functional role of human tumor necrosis factor receptors. Tumor Necrosis factor 1993, 4: 52
4. Grell, M et al; The type 1 receptor (CD120a) is the high-affinity receptor for soluble tumor necrosis factor. Proc Natl Acad Sci USA 1998, 95: 570
5. Krippner-Heidenreich, A et al; Single-chain TNF, a TNF derivative with enhanced stability and antitumoral activity. J Immunol 2008, 180: 8176
6. Kontermann, R et al; A humanized tumor necrosis factor receptor 1 (TNFR1)-specific antagonistic Antibody for selective inhibition of tumor necrosis factor (TNF) action. J Immunother 2008, 31: 225

NCBI and Uniprot Product Information

• NCBI GI #: 2290398
• NCBI GeneID: 282527
• Molecular Weight: 51,368 Da
• NCBI Official Full Name: tumor necrosis factor-receptor I
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 1A<
• NCBI Official Symbol: TNFRSF1A
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 1A; p55; p60; TNF-R1; TNF-RI; TNFR-I; tumor necrosis factor receptor 1; tumor necrosis factor receptor type I
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 1A
• UniProt Gene Name: TNFRSF1A
• UniProt Synonym Gene Names: TNFR1; TNF-R1; TNF-RI; TNFR-I
• UniProt Entry Name: TNR1A_BOVIN

Uniprot Description

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis ().