sTNF-RI Active Protein | TNR1A active protein

sTNF-RI, human recombinant

Cat. Num. AAA844530

• Gene Names: TNFRSF1A; FPF; MS5; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1-d2
• Purity: >98% by SDS-PAGE and HPLC analyses.
• Synonyms: sTNF-RI; human recombinant; Tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor 1; Tumor necrosis factor receptor type I; TNF-R1; TNF-RI; TNFR-I; p60; p55; CD120a; TNFRSF1A; TNFAR; TNFR1; FPF; TBP1; TNF-R; p55-R; TNFR55; TNFR60; TNF-R-I; TNF-R55; MGC19588;; TNR1A active protein

• Host: E. coli
• Purity/Purification: >98% by SDS-PAGE and HPLC analyses.
• Form/Format: Sterile filtered and lyophilized in 5 mM Tris pH 8.0

• Endotoxin Level: <0.1 ng per ug of sTNF-RI.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in 5 mM Tris, pH 8.0 to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers or stored at –4°C for 1 week or –20°C for future use.
• Biological Activity: The biological activity of sTNF-RI was determined by its inhibitory effect of the TNF-α mediated cytotoxicity in murine L-929 cells. The ED50 for the effect in the presence of 0.25 ng/ml of recombinant TNF-α is 0.05 ug/ml.
• Preparation and Storage: The lyophilized sTNF-RI is best-stored desiccated below 0°C. Reconstituted sTNF-RI should be stored at working aliquots at -20°C.

SDS-PAGE

(Purity of recombinant human sTNF-RI (2 ug) was analyzed by reduced SDS-PAGE electrophoresis.)

Related Product Information for TNR1A active protein

Tumor Necrosis Factor receptor type I (TNF-RI) is also referred as p60. The p60 form of the receptor is primarily expressed on epithelial cells. Recombinant Human soluble TNF receptor Type I (sTNF-RI) is a 18.3 kDa protein containing 162 amino acid residues comprising the extra cellular domain of TNF Receptor Type I.

NCBI and Uniprot Product Information

• NCBI GI #: 339760
• NCBI GeneID: 7132
• Molecular Weight: 18.3 kDa
• NCBI Official Full Name: TNF receptor
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 1A
• NCBI Official Symbol: TNFRSF1A
• NCBI Official Synonym Symbols: FPF; MS5; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1-d2
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 1A
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 1A
• UniProt Gene Name: TNFRSF1A
• UniProt Synonym Gene Names: TNFAR; TNFR1; TNF-R1; TNF-RI; TNFR-I; TBPI
• UniProt Entry Name: TNR1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease. [provided by RefSeq, Jul 2008]

Uniprot Description

TNF-R1: Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Binding of TNF to the extracellular domain leads to homotrimerization. The aggregated death domains provide a novel molecular interface that interacts specifically with the death domain of TRADD. Various TRADD-interacting proteins such as TRAFS, RIPK1 and possibly FADD, are recruited to the complex by their association with TRADD. This complex activates at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4, BRE, FEM1B, GRB2, SQSTM1 and TRPC4AP. Interacts with HCV core protein. Interacts with human cytomegalovirus/HHV-5 protein UL138. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: Golgi membrane; extracellular space; cell surface; mitochondrion; integral to plasma membrane; axon; extracellular region; plasma membrane; synapse; nucleus; cytosol; receptor complex; lipid raft

Molecular Function: protein binding; tumor necrosis factor receptor activity; protease binding; protein complex binding; tumor necrosis factor binding

Biological Process: response to alkaloid; viral reproduction; protein heterooligomerization; response to lipopolysaccharide; tumor necrosis factor-mediated signaling pathway; positive regulation of neuron apoptosis; response to wounding; negative regulation of interleukin-6 production; tetrapyrrole metabolic process; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of protein import into nucleus, translocation; cytokine and chemokine mediated signaling pathway; response to amino acid stimulus; positive regulation of tumor necrosis factor production; prostaglandin metabolic process; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of angiogenesis; response to ethanol; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; negative regulation of inflammatory response; defense response to bacterium; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; positive regulation of inflammatory response; negative regulation of apoptosis

Disease: Multiple Sclerosis, Susceptibility To, 5; Periodic Fever, Familial, Autosomal Dominant