anti-Mouse TNF-RI Monoclonal Antibody | anti-TNF-RI antibody
TNF-RI, Mouse, mAb 55R-170, FITC
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
TNF-RI; Monoclonal Antibody; Mouse; mAb 55R-170; FITC; TNFR type I; CD120a; TNF-R55; TNFRp55; p55-R; TNF receptor alpha chain; anti-TNF-RI antibody
Host
Armenian hamster
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Armenian hamster IgG
Form/Format
1 ml (100 ug/ml) 0.2 um filtered FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin.
Sequence Length
471
Applicable Applications for anti-TNF-RI antibody
Flow Cytometry (FC/FACS), FS, Immunoassay (IA), Immunoprecipitation (IP)
Application Notes
Application Use: For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Conjugate
FITC
Immunogen
Purified soluble extracellular domain of mouse TNF-RI
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-TNF-RI antibody
Product Description: The monoclonal antibody 55R-170 recognizes the extracellular part of mouse Tumor Necrosis Factor Receptor superfamily member 1A (TNF-RI), also known as CD120a or p55. TNF-RI belongs to the large TNF receptor family, among which TNF-RII (TNF-R p75-80), lymphotoxin-beta receptor (LTbetaR) and the Herpes virus entry mediator (HVEM). Ligands for these receptors belong to the Tumor Necrosis Factor (TNF) superfamily of cytokines, which activate signaling pathways for cell survival, death, and differentiation that orchestrate the development, organization and homeostasis of lymphoid, mammary, neuronal and ectodermal tissues.
TNF-RI contains a characteristic structural cassette termed death domain in its sequence that is conserved within a distinct subset of other TNF-R family members, such as CD95, DR3, DR4, and DR5. This death domain, was characterized as being essential for induction of apoptosis in vitro and has been structurally conserved within these TNF-R superfamily members. Deletion of the death domain of the TNF-RI results in a non-functional receptor, indicating that the death domain is required for the signal transduction of the physiological functions of TNF-RI in vivo.
TNF-RI is a 55 kD type I transmembrane protein and is expressed on a variety of cell types at low levels. It is considered to play a prominent role in cell stimulation by TNF-alpha. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is present as soluble form in body fluids (for instance plasma and CSF). This extracellular TNF-RI is generated by two mechanisms, namely proteolytic cleavage of TNF-RI ectodomains and release of full-length TNF-RI in the membranes of exosome-like vesicles. TNF-RI and TNF-RII both interact with the homomeric forms of LTbeta or TNF. However, TNF-RI functions as the high affinity receptor for soluble TNF (sTNF). TNF-RI has been shown to be involved in a wide variety of inflammatory diseases, among which neurodegenerative diseases (Parkinsons and Alzheimers disease), multiple sclerosis, asthma, atherosclerosis, rheumatology.
The monoclonal antibody 55R-170 also recognizes the soluble receptor.
TNF-RI contains a characteristic structural cassette termed death domain in its sequence that is conserved within a distinct subset of other TNF-R family members, such as CD95, DR3, DR4, and DR5. This death domain, was characterized as being essential for induction of apoptosis in vitro and has been structurally conserved within these TNF-R superfamily members. Deletion of the death domain of the TNF-RI results in a non-functional receptor, indicating that the death domain is required for the signal transduction of the physiological functions of TNF-RI in vivo.
TNF-RI is a 55 kD type I transmembrane protein and is expressed on a variety of cell types at low levels. It is considered to play a prominent role in cell stimulation by TNF-alpha. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is present as soluble form in body fluids (for instance plasma and CSF). This extracellular TNF-RI is generated by two mechanisms, namely proteolytic cleavage of TNF-RI ectodomains and release of full-length TNF-RI in the membranes of exosome-like vesicles. TNF-RI and TNF-RII both interact with the homomeric forms of LTbeta or TNF. However, TNF-RI functions as the high affinity receptor for soluble TNF (sTNF). TNF-RI has been shown to be involved in a wide variety of inflammatory diseases, among which neurodegenerative diseases (Parkinsons and Alzheimers disease), multiple sclerosis, asthma, atherosclerosis, rheumatology.
The monoclonal antibody 55R-170 also recognizes the soluble receptor.
References
1. Sheehan, K et al; Monoclonal antibodies specific for murine p55 and p75 tumor necrosis factor receptors: identification of a novel in vivo role for p75. J Exp Med 1995, 181: 607 2. Pinckard, J et al; Ligand-induced formation of p55 and p75 tumor necrosis factor receptor heterocomplexes on intact cells. J Biol Chem 1997, 272: 10784
3. Ji, H et al; Critical roles for interleuking 1 and tumor necrosis factor alpha in antibody-induced arthritis. J Exp Med 2002, 196: 77
4. Lee, S et al; Inhibition of TCR-induced CD8 T cell death by IL-12: regulation of Fas ligand and cellular FLIP expression and caspase activation by IL-12. J Immunol 2003, 170: 2456
3. Ji, H et al; Critical roles for interleuking 1 and tumor necrosis factor alpha in antibody-induced arthritis. J Exp Med 2002, 196: 77
4. Lee, S et al; Inhibition of TCR-induced CD8 T cell death by IL-12: regulation of Fas ligand and cellular FLIP expression and caspase activation by IL-12. J Immunol 2003, 170: 2456
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
51,368 Da
NCBI Official Full Name
tumor necrosis factor-receptor I
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 1A<
NCBI Official Symbol
TNFRSF1A
NCBI Protein Information
tumor necrosis factor receptor superfamily member 1A; p55; p60; TNF-R1; TNF-RI; TNFR-I; tumor necrosis factor receptor 1; tumor necrosis factor receptor type I
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 1A
UniProt Gene Name
TNFRSF1A
UniProt Synonym Gene Names
TNFR1; TNF-R1; TNF-RI; TNFR-I
UniProt Entry Name
TNR1A_BOVIN
Uniprot Description
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis ().
Research Articles on TNF-RI
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Product Notes
The TNF-RI tnfrsf1a (Catalog #AAA584391) is an Antibody produced from Armenian hamster and is intended for research purposes only. The product is available for immediate purchase. The TNF-RI, Mouse, mAb 55R-170, FITC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TNF-RI can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FS, Immunoassay (IA), Immunoprecipitation (IP). Application Use: For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the TNF-RI tnfrsf1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNF-RI, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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