Rat anti-Mouse TNF-RI Monoclonal Antibody | anti-TNF-RI antibody

TNF-RI, Mouse, mAb FITC

Cat. Num. AAA584092

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Synonyms: TNF-RI; Monoclonal Antibody; Mouse; mAb FITC; CD120a; Tumor necrosis factor receptor superfamily member 1A; p55/p60; anti-TNF-RI antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 1% bovine serum albumin and 0.02 % sodium azide.
• Sequence Length: 471

• Applicable Applications for anti-TNF-RI antibody: F, Flow Cytometry (FC/FACS), Immunoassay (IA)
• Application Notes: Application Notes: The monoclonal antibody HM104 can be used for immuno assay, and flow cytometry. Furthermore, the monoclonal antibody HM104 is useful for immuno precipitation and immunohistology on frozen sections. Be aware that the reactivity of the antibody with soluble TNF-Receptor is inhibited by high concentrations of both mouse and human TNF-alpha.; Application Use: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Conjugate: FITC
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-TNF-RI antibody

Product Description: The monoclonal antibody HM104 recognizes the extracellular part of the Tumor Necrosis Factor Receptor type I (TNF-RI) of the membrane-bound as well as the soluble receptor. TNF-RI (~55-60 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha. TNF-RI signaling is initiated when trimeric TNF-alpha binds TNF-RI receptors. Subsequent TNF-RI trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD), Receptor Interacting Protein (RIP), cellular Inhibitor of Apoptosis Protein 1 (cIAP1), TNF Receptor Associated Factor 2 (TRAF2), and likely TRAF5. Studies with TNF-RI-deficient mice indicate that TNF-RI mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways.

References

1. Bigini, P et al; Immunohistochemical localization of TNFalpha and its receptors in the rodent central nervous system. Methods Mol Med 2004, 98: 73 2. Mennini, T et al; Glial activation and TNFR-I upregulation precedes motor dysfunction in the spinal cord of mnd mice. Cytokine 2004, 25: 127
3. Ghezzi, P et al; Tumor necrosis factor as a pharmacological target. Methods Mol Med 2004, 98: 1

NCBI and Uniprot Product Information

• NCBI GI #: 2290398
• NCBI GeneID: 282527
• Molecular Weight: 51,368 Da
• NCBI Official Full Name: tumor necrosis factor-receptor I
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 1A<
• NCBI Official Symbol: TNFRSF1A
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 1A; p55; p60; TNF-R1; TNF-RI; TNFR-I; tumor necrosis factor receptor 1; tumor necrosis factor receptor type I
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 1A
• UniProt Gene Name: TNFRSF1A
• UniProt Synonym Gene Names: TNFR1; TNF-R1; TNF-RI; TNFR-I
• UniProt Entry Name: TNR1A_BOVIN

Uniprot Description

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis ().