Mouse anti-Human TNF ALPHA Monoclonal Antibody | anti-TNF ALPHA antibody
MOUSE ANTI HUMAN TNF ALPHA:FITC
Gene Names
TNF; DIF; TNFA; TNFSF2; TNF-alpha
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
TNF ALPHA; Monoclonal Antibody; MOUSE ANTI HUMAN TNF ALPHA:FITC; anti-TNF ALPHA antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
B-D9
Specificity
TNF ALPHA
Clone B-D9 specifically recognizes human TNF-alpha, both natural and recombinant forms. The FITC conjugated reagent is designed for the detection and enumeration of TNF-alpha expressing cells in flow cytometric assays following cell membrane permeabilisation.
Clone B-D9 specifically recognizes human TNF-alpha, both natural and recombinant forms. The FITC conjugated reagent is designed for the detection and enumeration of TNF-alpha expressing cells in flow cytometric assays following cell membrane permeabilisation.
Form/Format
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC))
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Sequence Length
233
Applicable Applications for anti-TNF ALPHA antibody
Flow Cytometry (FACS)
Application Notes
Flow Cytometry: Max Dilution: Neat
Application Note: Membrane permeabilisation is required for this application. We recommend the use of Leucoperm for this purpose.
Flow Cytometry: Use 10ul of the suggested working dilution to label 5 x 105 cells in 50ul
Application Note: Membrane permeabilisation is required for this application. We recommend the use of Leucoperm for this purpose.
Flow Cytometry: Use 10ul of the suggested working dilution to label 5 x 105 cells in 50ul
Immunogen
Recombinant human TNF-alpha
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63 Ag8.653 myeloma cell line
Preparation
Purified IgG prepared by ion exchange chromatography
Preservative Stabilizers
0.1% Sodium Azide (NaN3)
5% Bovine Serum Albumin
5% Bovine Serum Albumin
Buffer Solution
Phosphate buffered saline
Preparation and Storage
Store at +4 degree C or at -20 degree C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of dispatch
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of dispatch
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,644 Da
NCBI Official Full Name
tumor necrosis factor
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
DIF; TNFA; TNFSF2; TNF-alpha
NCBI Protein Information
tumor necrosis factor; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_HUMAN
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Product Notes
The TNF ALPHA tnf (Catalog #AAA210677) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN TNF ALPHA:FITC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TNF ALPHA can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FACS). Flow Cytometry: Max Dilution: Neat Application Note: Membrane permeabilisation is required for this application. We recommend the use of Leucoperm for this purpose. Flow Cytometry: Use 10ul of the suggested working dilution to label 5 x 105 cells in 50ul. Researchers should empirically determine the suitability of the TNF ALPHA tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNF ALPHA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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