Mouse anti-Human TNF-alpha Monoclonal Antibody | anti-TNF-alpha antibody
TNF-alpha (APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand Superfamily membe
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
TNF-alpha; Monoclonal Antibody; TNF-alpha (APC1; Cachectin; Differentiation inducing factor (DIF); Macrophage cytotoxic factor (MCF); Necrosin; TNF alpha; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand Superfamily membe; APC1; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precursor; anti-TNF-alpha antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4C6-H8
Specificity
Recognizes human TNF-alpha. Species Crossreactivity: mouse, rat, rabbit, feline, canine, and zebrafish.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-TNF-alpha antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC) Frozen/Paraffin
Application Notes
FC/FACS: 0.5-1ug/million cells
IHC-F/P: 1-2ug/ml for 30 min at RT
IP: 1-2ug/500ug protein
IF: 1-2ug/ml
Applications are based on unconjugated antibody.
IHC-F/P: 1-2ug/ml for 30 min at RT
IP: 1-2ug/500ug protein
IF: 1-2ug/ml
Applications are based on unconjugated antibody.
Immunogen
A hexadecapeptide corresponding to aa115-130 of human TNF-alpha, conjugated to thyroglobulin. Cellular Localization: Cytoplasmic and extracellular (secreted)
Conjugate
MaxLight490
Positive Control
HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Product Categories/Family for anti-TNF-alpha antibody
References
1. Bebok Z; Markus B; Nemeth P. Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry. Breast Cancer Research and Treatment, 1994, 29(3):229-35. 2. Bebok Z; Szekeres G; Horvath G; Duda E; Nemeth P. [Creation of monoclonal antibodies against tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor alpha (TFG-alpha), their definition and possible use]. Orvosi Hetilap, 1993 Jun 13, 134(24):1303-7. Language: Hungarian.
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Product Notes
The TNF-alpha (Catalog #AAA6204535) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TNF-alpha (APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand Superfamily membe reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TNF-alpha can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC) Frozen/Paraffin. FC/FACS: 0.5-1ug/million cells IHC-F/P: 1-2ug/ml for 30 min at RT IP: 1-2ug/500ug protein IF: 1-2ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TNF-alpha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNF-alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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