Mouse anti-Human TLR6 Monoclonal Antibody | anti-TLR6 antibody

TLR6, Human, mAb TLR6.127, FITC

Cat. Num. AAA584316

• Gene Names: TLR6; CD286
• Reactivity: Human
• Applications: Immunofluorescence
• Synonyms: TLR6; Monoclonal Antibody; Human; mAb TLR6.127; FITC; CD286; anti-TLR6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1.0 ml (100 ug/ml) 0.2 um filtered FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
• Sequence Length: 796

• Applicable Applications for anti-TLR6 antibody: Immunofluorescence (IF)
• Application Notes: Application Use: For immunofluorescence staining and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Conjugate: FITC
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-TLR6 antibody

Product Description: The monoclonal antibody TLR6.127 reacts with the human Toll-like receptor 6 (TLR6). Toll-like receptors (TLRs) are highly conserved throughout evolution. They play an essential role in initiating the innate immune response against infectious pathogens. In Drosophila, toll is required for anti-fungal response, while the related 18-wheeler is involved in antibacterial defence. In humans, ten members of the TLR family protein (TLR1 to TLR10) have been identified. TLRs recognize a wide variety of pathogen-associated molecular patterns from bacteria, viruses, and fungi and elicit a wide array of antimicrobial responses. Among TLRs, TLR6 is expressed on the cell surface of monocytes, monocyte-derived immature dendritic cells (iDCs), and neutrophils, but not on B, T or natural killer (NK) cells. Human TLR6 is a 796-aa type l transmembrane protein that is 74% identical with mouse. It contains an N-terminal signal peptide, 19 tandemly repeated extracellular leucine-rich motifs, and a cytoplasmic domain called Toll/IL-1R homology domain, as seen in other TLRs. TLR6 function has been studied mainly in mouse cells. Constitutive expression of TLR6 activates both the nuclear factor kappa-B (NFK-B) and Jun N-terminal kinase (JNK) pathways. Studies in human cells revealed that TLR6 and TLR2 colocalize at the plasma membrane of monocytes. Human TLR6 recognizes diacylated lipoprotein and peptidoglycan at the cell surface cooperatively with human TLR2. Thus, coexpression of TLR2 and TLR6 at the cell surface is crucial for recognition of diacylated lipopeptide and peptideoglycan and subsequent cellular activation in human cells.

References

Nakoa, Y et al; Surface-expressed TLR6 participates in the recognition of diacylated lipopeptide and peptidoglycan in human cells. J Immunol 2005, 174: 1566.

NCBI and Uniprot Product Information

• NCBI GI #: 20143971
• NCBI GeneID: 10333
• NCBI Accession #: NP_006059.2
• NCBI GenBank Nucleotide #: NM_006068.4
• Molecular Weight: 91,880 Da
• NCBI Official Full Name: toll-like receptor 6
• NCBI Official Synonym Full Names: toll-like receptor 6
• NCBI Official Symbol: TLR6
• NCBI Official Synonym Symbols: CD286
• NCBI Protein Information: toll-like receptor 6
• UniProt Protein Name: Toll-like receptor 6
• UniProt Gene Name: TLR6
• UniProt Entry Name: TLR6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor functionally interacts with toll-like receptor 2 to mediate cellular response to bacterial lipoproteins. A Ser249Pro polymorphism in the extracellular domain of the encoded protein may be associated with an increased of asthma is some populations.[provided by RefSeq, Jan 2011]

Uniprot Description

TLR6: Participates in the innate immune response to Gram- positive bacteria and fungi. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. Belongs to the Toll-like receptor family.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: phagocytic vesicle membrane; integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity; protein heterodimerization activity; receptor activity; diacylated lipoprotein binding

Biological Process: positive regulation of JNK activity; T-helper 1 type immune response; regulation of cytokine secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; detection of diacylated bacterial lipoprotein; positive regulation of interleukin-6 biosynthetic process; activation of NF-kappaB-inducing kinase; signal transduction; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 6 signaling pathway; defense response to bacterium; toll-like receptor signaling pathway; innate immune response; immune response; inflammatory response; toll-like receptor 4 signaling pathway