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Rat anti-Mouse TLR4/MD-2 Monoclonal Antibody | anti-TLR4/MD-2 antibody

TLR4/MD-2, Mouse, mAb MTS510

Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
TLR4/MD-2; Monoclonal Antibody; Mouse; mAb MTS510; Toll-like receptor 4:- TLR4; CD284; ARMD10; anti-TLR4/MD-2 antibody
Ordering
For Research Use Only!
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS and 0.1% bovine serum albumin.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-TLR4/MD-2 antibody
F, Flow Cytometry (FC/FACS), FS, Immunoprecipitation (IP)
Application Notes
Application Notes: F: 6 um acetone fixed sections blocked with 0.3% hydrogen peroxidase in methanol and subsequently with normal serum. Sections were incubated for 2h at RT at 1/100 dilution of mAb
Application Use: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Immunogen
Ba/F3 cells expressing mouse TLR4 and MD-22
Positive Control
RAW264.7 cells
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-TLR4/MD-2 antibody
Product Description: The monoclonal antibody MTS510 reacts with the Toll-like receptor 4 (TLR4, CD284) that is associated with MD2. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single alpha-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses. Toll-like receptor 4 (TLR4; CD284) has been identified, next to MD-2 and CD14, as a receptor that is central to the innate immune response to LPS of Gram-negative bacteria. TLR4 is unique among TLRs in its ability to activate two distinct signaling pathways; one pathway is activated by the adaptors TIRAP (Toll/interleukin-1- receptor (TIR)-domain-containing adaptor protein) and MyD88, which leads to the induction of pro'inflammatory cytokines. The second pathway is activated by the adaptors TRIF (TIR-domaincontaining adaptor protein inducing interferon'beta) and TRAM (TRIFrelated adaptor molecule), which leads to the induction of type I interferons. MD-2 exists as a cell surface protein in association with TLR4. It also exists as secreted forms consisting of MD-2 monomer and multimers. Circulating sMD-2 is mainly present as a doublet of ~20 and 25 kD, representing differentially glycosylated forms. Unlike TLR4, sMD-2 binds directly LPS without the need of soluble CD14 (sCD14). However, LPS-MD-2 interactions are increased when LPS is pretreated with CD14. Only monomeric sMD-2 is biologically active and able to associate with TLR4 and LPS. sMD-2 circulates in plasma of healthy individuals as a non-active, polymeric protein. In septic plasma, the total amount of sMD-2 was strongly elevated and contained both sMD-2 polymers and monomers. Soluble MD-2 is proposed to be an important mediator of organ inflammation during sepsis. During experimental human endotoxemia, the monomeric and total sMD-2 content in plasma increased with the kinetics of an acute phase protein. This parallels enhanced TLR4 costimulatory activity. In vitro studies revealed that sMD-2 release appears to be restricted to endothelial and dendritic cells. The monoclonal antibody MTS510 reacts preferentially, especially in flow cytometry, with mouse TLR4 that is associated with MD-2. MTS510 is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to coprecipitate MD-2 (30 kDa) with TLR4 (100 kDa).
References
1. Nomura, F et al ; Cutting edge: Endotoxin tolerance in mouse peritoneal macrophages correlate with down-regulation of surface toll-like receptor 4 expression. J Immunol 2000, 164: 3476 2. Akashi, S et al; Cutting edge: Cell surface expression and lipopolysaccharide signalling via the toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages. J Immunol 2000, 164: 3471
3. Sato, S et al; Synergy and cross-tolerance between toll-like receptor (TLR)2- and TLR4-mediated signaling pathways. J Immunol 2000, 165: 7096
4. Ortega-Cava, C et al; Strategic compartmentalization of toll-like receptor 4 in the mouse gut. J Immunol 2003, 170: 3977
5. Tsujimoto, H. A critical role of CpG motifs in a murine peritonitis model by their binding to highly expressed toll-like receptor-9 on liver NTK cells. J hepatology 2006, 45: 836
6. Cook,C et al. Lipopolysaccharide, Tumor Necrosis Factor alpha, or Interleukin-1β triggers reactivation of latent CMV in immunocompetent mice. J virology 2006, 80: 9151
7. Tsukumo_D et al. Loss-of-function mutation in Toll-like receptor 4 prevents diet-induced obesity and insulin resistance. Diabetes 2007, 56: 1986
8. Burton, O et al. Importance of TLR2 in the direct response of T lymphocytes to Schistosoma mansoni antigens. Eur J Immunol 2010, 40: 2221

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Product Notes

The TLR4/MD-2 (Catalog #AAA584221) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The TLR4/MD-2, Mouse, mAb MTS510 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TLR4/MD-2 can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), FS, Immunoprecipitation (IP). Application Notes: F: 6 um acetone fixed sections blocked with 0.3% hydrogen peroxidase in methanol and subsequently with normal serum. Sections were incubated for 2h at RT at 1/100 dilution of mAb Application Use: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the TLR4/MD-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLR4/MD-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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