Western Blot (WB)
(Western blot analysis of TLR2. Anti- TLR2 antibody (Clone: ABM3A87) was used at 2 µg/ml on mouse embryonic liver lysate.)
Mouse anti-Mouse, Human TLR2 Monoclonal Antibody | anti-TLR2 antibody
TLR2 Monoclonal Antibody
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
FACS analysis: 0.5 ug/10^6 cells
Immunohistochemical analysis: 5 ug/ml
Western Blot (WB)
(Western blot analysis of TLR2. Anti- TLR2 antibody (Clone: ABM3A87) was used at 2 µg/ml on mouse embryonic liver lysate.)
Western Blot (WB)
(Western blot analysis of TLR2. Anti- TLR2 antibody (Clone: ABM3A87) was used at 2 µg/ml on mouse embryonic liver lysate.)
Flow Cytometry (FC/FACS)
(Fig-3: Intracellular flow analysis of TLR2 in PBMC (Monocytes) using 0.5 µg/10^6 cells of TLR2 antibody (Clone: ABM3A87). Green represents isotype control; red represents anti-TLR2 antibody. Goat anti-Mouse PE conjugate was used as secondary.)
Flow Cytometry (FC/FACS)
(Fig-3: Intracellular flow analysis of TLR2 in PBMC (Monocytes) using 0.5 µg/10^6 cells of TLR2 antibody (Clone: ABM3A87). Green represents isotype control; red represents anti-TLR2 antibody. Goat anti-Mouse PE conjugate was used as secondary.)
Flow Cytometry (FC/FACS) #2
(Fig-4: Intracellular flow analysis of TLR2 in THP-1 cells using 0.5 µg/10^6 cells of TLR2 antibody (Clone: ABM3A87). Green represents isotype control; red represents anti- TLR2 antibody. Goat anti-Mouse PE conjugate was used as secondary antibody.)
Flow Cytometry (FC/FACS) #2
(Fig-4: Intracellular flow analysis of TLR2 in THP-1 cells using 0.5 µg/10^6 cells of TLR2 antibody (Clone: ABM3A87). Green represents isotype control; red represents anti- TLR2 antibody. Goat anti-Mouse PE conjugate was used as secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of TLR2 in normal human prostate tissue using TLR2 antibody (Clone: ABM3A87) at 5 ug/ml.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of TLR2 in normal human prostate tissue using TLR2 antibody (Clone: ABM3A87) at 5 ug/ml.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq, Jul 2008]
Uniprot Description
TLR2: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage- activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. Interacts with LY96, TLR1 and TLR6 (via extracellular domain). Binds MYD88 (via TIR domain). Interacts with TICAM1. Ligand binding induces the formation of a heterodimer with TLR1. Interacts with CNPY3. Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. Belongs to the Toll-like receptor family.
Protein type: Cell surface; Membrane protein, integral; Receptor, misc.; Apoptosis; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 4q32
Cellular Component: cell surface; cell projection; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane
Molecular Function: protein binding; peptidoglycan binding; transmembrane receptor activity; triacylated lipoprotein binding; protein heterodimerization activity; lipopolysaccharide receptor activity; receptor activity; pattern recognition receptor activity; diacylated lipoprotein binding
Biological Process: positive regulation of nitric oxide biosynthetic process; response to toxin; positive regulation of leukocyte migration; positive regulation of NF-kappaB import into nucleus; leukotriene metabolic process; detection of triacylated bacterial lipoprotein; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of interferon-beta production; positive regulation of oligodendrocyte differentiation; toll-like receptor 4 signaling pathway; negative regulation of interleukin-12 production; detection of diacylated bacterial lipoprotein; cell surface pattern recognition receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; defense response to Gram-positive bacterium; myelin formation in the central nervous system; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; I-kappaB phosphorylation; positive regulation of nitric-oxide synthase biosynthetic process; apoptosis; positive regulation of interleukin-12 production; microglial cell activation; response to molecule of fungal origin; positive regulation of interleukin-18 production; signal transduction; nitric oxide metabolic process; response to insulin stimulus; positive regulation of interleukin-8 production; negative regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; inflammatory response; positive regulation of Wnt receptor signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of toll-like receptor signaling pathway; induction by symbiont of defense-related host nitric oxide production; MyD88-dependent toll-like receptor signaling pathway; negative regulation of interleukin-17 production; toll-like receptor signaling pathway; response to hypoxia; innate immune response; immune response; chloramphenicol transport; positive regulation of inflammatory response; positive regulation of cytokine secretion
Disease: Leprosy, Susceptibility To, 3; Mycobacterium Tuberculosis, Susceptibility To
Research Articles on TLR2
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Product Notes
The TLR2 tlr2 (Catalog #AAA668018) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TLR2 Monoclonal Antibody reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's TLR2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC), Western Blot (WB), Immunofluorescence (IF). Western blot analysis: 2-4 ug/ml FACS analysis: 0.5 ug/10^6 cells Immunohistochemical analysis: 5 ug/ml. Researchers should empirically determine the suitability of the TLR2 tlr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLR2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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