Mouse anti-Human TIM50 Monoclonal Antibody | anti-TIM50 antibody

TIM50 (680) Mouse mAb

Cat. Num. AAA435157

• Gene Names: TIMM50; MGCA9; TIM50; TIM50L
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Synonyms: TIM50; Monoclonal Antibody; TIM50 (680) Mouse mAb; TIMM50(680) Mouse mAb; homolog of yeast Tim50; MGC102733; Mitochondrial import inner membrane translocase subunit TIM50; Tim50-like protein; TIM50L; TIMM50; translocase of inner mitochondrial membrane 50 homolog; translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae); translocase of inner mitochondrial membrane 50 homolog (yeast); PRO1512; anti-TIM50 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 456

• Applicable Applications for anti-TIM50 antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Source Note: This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human TIM50
• Preparation and Storage: Store at -20 degree C. Stable for one year from the date of shipment.

Western Blot (WB)

Immunoprecipitation (IP)

(TIM50 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25108 of 10 ug. Western blot was performed from the immunopre-cipitate using M25108 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 40 KDaObserved band size : 40 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling TIM50 with M25108 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)

Related Product Information for anti-TIM50 antibody

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.

Product Categories/Family for anti-TIM50 antibody

Cell Marker; Subcellular Marker; Membrane

NCBI and Uniprot Product Information

• NCBI GI #: 48526509
• NCBI GeneID: 92609
• NCBI Accession #: NP_001001563.1
• NCBI GenBank Nucleotide #: NM_001001563.3
• UniProt Accession #: Q3ZCQ8; Q330K1; Q6QA00; Q96FJ5; Q96GY2; Q9H370
• Molecular Weight: Predicted MW: 40kDa
• NCBI Official Full Name: mitochondrial import inner membrane translocase subunit TIM50 isoform 1
• NCBI Official Synonym Full Names: translocase of inner mitochondrial membrane 50
• NCBI Official Symbol: TIMM50
• NCBI Official Synonym Symbols: MGCA9; TIM50; TIM50L
• NCBI Protein Information: mitochondrial import inner membrane translocase subunit TIM50
• UniProt Protein Name: Mitochondrial import inner membrane translocase subunit TIM50
• UniProt Gene Name: TIMM50
• UniProt Synonym Gene Names: TIM50

NCBI Description

This gene encodes a subunit of the TIM23 inner mitochondrial membrane translocase complex. The encoded protein functions as the receptor subunit that recognizes the mitochondrial targeting signal, or presequence, on protein cargo that is destined for the mitochondrial inner membrane and matrix. This protein may also play a role in maintaining the membrane permeability barrier, and knockdown of this gene in human cells results in the release of cytochrome c and apoptosis. [provided by RefSeq, Jul 2016]

Uniprot Description

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo.