TIGIT recombinant protein

Recombinant human TIGIT Protein with C-His tag

Cat. Num. AAA188177

• Gene Names: TIGIT; VSIG9; VSTM3; WUCAM
• Applications: Western Blot
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Synonyms: TIGIT; Recombinant human TIGIT Protein with C-His tag; Human TIGIT Protein; His Tag; TIGIT;VSIG9;VSTM3; TIGIT recombinant protein

• Host: HEK293 cells
• Purity/Purification: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Form/Format: Lyophilized from sterile PBS, pH 7.4. Normally 5%-117% trehalose is added as protectants before lyophilization.
• Sequence: TIGIT(Met22-Pro141)

• Applicable Applications for TIGIT recombinant protein: Western Blot (WB)
• Source: Human
• Conjugation: C-His tag
• Reconstitution: Reconstitute with deionized water
• Preparation and Storage: Store at -80 degree C for 12 months (Avoid repeated freezing and thawing); Shipping: Blue Ice

SDS-Page

(Figure 1. Human TIGIT Protein, His Tag on SDS-PAGE under reducing condition.)

ELISA (EIA)

(Figure 2. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human TIGIT, His tagged protein can bind Anti-TIGIT Neutralizing antibody in a linear range of 0.13-16.0 ng/ml.)

Related Product Information for TIGIT recombinant protein

This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.

NCBI and Uniprot Product Information

• NCBI GI #: 121943253
• NCBI GeneID: 201633
• UniProt Accession #: Q495A1; Q495A3; Q5JPD8; Q6MZS2; Q8N877
• Molecular Weight: 18,432 Da
• NCBI Official Full Name: T-cell immunoreceptor with Ig and ITIM domains
• NCBI Official Synonym Full Names: T cell immunoreceptor with Ig and ITIM domains
• NCBI Official Symbol: TIGIT
• NCBI Official Synonym Symbols: VSIG9; VSTM3; WUCAM
• NCBI Protein Information: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Protein Name: T-cell immunoreceptor with Ig and ITIM domains
• Protein Family: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Gene Name: TIGIT
• UniProt Synonym Gene Names: VSIG9; VSTM3

NCBI Description

This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009]

Uniprot Description

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.