Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Placenta stained with Thymidine Phosphorylase / PD-ECGF Monoclonal Antibody (P-GF.44C).)
Mouse Thymidine Phosphorylase/PD-ECGF Monoclonal Antibody | anti-TYMP antibody
Thymidine Phosphorylase/PD-ECGF (Angiogenesis Marker)
Gene Names
TYMP; TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
Reactivity
Human, Mouse, Rat. Others not known.
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Synonyms
Thymidine Phosphorylase/PD-ECGF; Monoclonal Antibody; Thymidine Phosphorylase/PD-ECGF (Angiogenesis Marker); ECGF; ECGF1; Gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PD-ECGF; PDECGF; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine phosphorylase; TP; Tymp; anti-TYMP antibody
Host
Mouse
Reactivity
Human, Mouse, Rat. Others not known.
Clonality
Monoclonal
Isotype
IgG1
Clone Number
P-GF.44C
Specificity
Recognizes a protein (amino acid 482) of 55kDa (in vivo 110kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
482
Applicable Applications for anti-TYMP antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) Formalin
Application Notes
WB: 0.5-1ug/ml
IP: 0.5-1ug/500ug protein lysate
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
IP: 0.5-1ug/500ug protein lysate
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunogen
Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein
Cellular Localization
Cytoplasmic and Nuclear
Hu-chromosome Location
22q13.33
Positive Control
HUVEC cells. Breast, Bladder, Lung or Kaposi tumors
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Placenta stained with Thymidine Phosphorylase / PD-ECGF Monoclonal Antibody (P-GF.44C).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Placenta stained with Thymidine Phosphorylase / PD-ECGF Monoclonal Antibody (P-GF.44C).)
Immunohistochemistry (IHC)
(Formalin-paraffin human Pancreas stained with Chromogranin A MAb (CHGA/798))
Immunohistochemistry (IHC)
(Formalin-paraffin human Pancreas stained with Chromogranin A MAb (CHGA/798))
References
Fox SB, et. al. Journal of Pathology, 1995, 176:183-90. O'Brien TS, et. al. Cancer Research, 1996, 56(20):4799-804. Fox SB, et. al. British Journal of Cancer, 1996, 73:275-80
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55kDa
NCBI Official Full Name
thymidine phosphorylase isoform 1
NCBI Official Synonym Full Names
thymidine phosphorylase
NCBI Official Symbol
TYMP
NCBI Official Synonym Symbols
TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
NCBI Protein Information
thymidine phosphorylase
UniProt Protein Name
Thymidine phosphorylase
UniProt Gene Name
TYMP
UniProt Synonym Gene Names
ECGF1; TP; PD-ECGF
NCBI Description
This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]
Uniprot Description
May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro.
Research Articles on TYMP
Similar Products
Product Notes
The TYMP tymp (Catalog #AAA439920) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Thymidine Phosphorylase/PD-ECGF (Angiogenesis Marker) reacts with Human, Mouse, Rat. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's Thymidine Phosphorylase/PD-ECGF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) Formalin. WB: 0.5-1ug/ml IP: 0.5-1ug/500ug protein lysate IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the TYMP tymp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thymidine Phosphorylase/PD-ECGF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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