Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CASC3 Antibody (Y181) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit CASC3 Polyclonal Antibody | anti-CASC3 antibody
CASC3 Antibody (Y181)
WB~~1:1000
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CASC3 Antibody (Y181) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CASC3 Antibody (Y181) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB)
(Western blot analysis of anti-CASC3 Antibody (Y181) in NCI-H460 cell line lysates (35ug/lane). CASC3(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-CASC3 Antibody (Y181) in NCI-H460 cell line lysates (35ug/lane). CASC3(arrow) was detected using the purified Pab.)
Degot,S., Oncogene 21 (28), 4422-4434 (2002)
NCBI and Uniprot Product Information
NCBI Description
The product of this gene is a core component of the exon junction complex (EJC), a protein complex that is deposited on spliced mRNAs at exon-exon junctions and functions in nonsense-mediated mRNA decay (NMD). The encoded protein binds RNA and interacts with two other EJC core components. It is predominantly located in the cytoplasm, but shuttles into the nucleus where it localizes to nuclear speckles. [provided by RefSeq, Jul 2008]
Uniprot Description
CASC3: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Stimulates the ATPase and RNA- helicase activities of EIF4A3. Plays a role in the stress response by participating in cytoplasmic stress granules assembly and by favoring cell recovery following stress. Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Binds poly(G) and poly(U) RNA homopolymer. Belongs to the CASC3 family.
Protein type: Translation; RNA-binding
Chromosomal Location of Human Ortholog: 17q21.1
Cellular Component: perinuclear region of cytoplasm; nuclear speck; cytosol; ribonucleoprotein complex
Molecular Function: identical protein binding; protein binding; enzyme binding; ubiquitin protein ligase binding
Biological Process: regulation of translation; intracellular mRNA localization; mRNA transport; RNA splicing; mRNA catabolic process, nonsense-mediated decay; gene expression; mRNA processing
Research Articles on CASC3
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Product Notes
The CASC3 casc3 (Catalog #AAA9214800) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 159-188. The CASC3 Antibody (Y181) reacts with Human (Predicted Reactivity: Bovine, Zebrafish, Mouse, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's CASC3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). IHC~~1:10~50 WB~~1:1000. Researchers should empirically determine the suitability of the CASC3 casc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CASC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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