Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Thioredoxin2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HepG2 cell lysate Lane 2 : HeLa cell lysate Lane 3 : Raji cell lysate Lane 4 : K562 cell lysate Lane 5 : Jurkat cell lysate Lane 6 : A431 cell lysate Lane 7 : MCF7 cell lysate Lane 8 : THP1 cell lysate Recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Thioredoxin2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant human Thioredoxin1 protein Lane 2 : Recombinant human Thioredoxin2 protein)
Mouse anti-Human Thioredoxin2 Monoclonal Antibody | anti-TXN2 antibody
Thioredoxin2 antibody
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Thioredoxin2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HepG2 cell lysate Lane 2 : HeLa cell lysate Lane 3 : Raji cell lysate Lane 4 : K562 cell lysate Lane 5 : Jurkat cell lysate Lane 6 : A431 cell lysate Lane 7 : MCF7 cell lysate Lane 8 : THP1 cell lysate Recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Thioredoxin2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant human Thioredoxin1 protein Lane 2 : Recombinant human Thioredoxin2 protein)
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Thioredoxin2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HepG2 cell lysate Lane 2 : HeLa cell lysate Lane 3 : Raji cell lysate Lane 4 : K562 cell lysate Lane 5 : Jurkat cell lysate Lane 6 : A431 cell lysate Lane 7 : MCF7 cell lysate Lane 8 : THP1 cell lysate Recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Thioredoxin2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant human Thioredoxin1 protein Lane 2 : Recombinant human Thioredoxin2 protein)
Immunofluorescence (IF)
(ICC/IF analysis of TXN2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXN2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of TXN2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXN2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
NCBI and Uniprot Product Information
NCBI Description
This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. [provided by RefSeq, Jul 2008]
Uniprot Description
Important for the control of mitochondrial reactive oxygen species homeostasis, apoptosis regulation and cell viability. Possesses a dithiol-reducing activity.
Research Articles on TXN2
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Product Notes
The TXN2 txn2 (Catalog #AAA200433) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Thioredoxin2 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Thioredoxin2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the TXN2 txn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thioredoxin2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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