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Mouse anti-Marmoset THIOREDOXIN Monoclonal Antibody | anti-TXN antibody

MOUSE ANTI HUMAN THIOREDOXIN

Gene Names
TXN; TRX; TRDX; TRX1
Reactivity
Marmoset
Applications
Immunohistochemistry, ELISA, Immunohistochemistry, Western Blot
Synonyms
THIOREDOXIN; Monoclonal Antibody; MOUSE ANTI HUMAN THIOREDOXIN; anti-TXN antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Marmoset
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2B1
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
85
Applicable Applications for anti-TXN antibody
Immunohistology Frozen, ELISA (EIA), Immunohistology Paraffin, Western Blot (WB)
Application Notes
Western Blotting: Minimum Dilution: 10ug/ml; Maximum Dilution: 50ug/ml
Perservative Stabilisers
0.09% Sodium Azide
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-TXN antibody
Mouse anti Human thioredoxin antibody, clone 2B1 recognizes human thioredoxin, also known as ATL-derived factor or Surface-associated sulphydryl protein. Thioredoxin is a 105 amino acid ~12kDa dithiol oxidoreductase with powerful protein disulphide oxidoreductase activity containing a single thioredoxin domain (UniProt: P10599). Thioredoxin is involved in a number of cellular processes, including activation of the transcription factor NF-KB (Kelleher et al. 2014). Thioredoxin is highly expressed in the glandular cells of the GI tract and uterine tissues, also activated B and T-cells (Rubartelli et al. 1992). Mouse anti Human thioredoxin antibody, clone 2B1 has been used successfully in a biotinylated format as a detection reagent in a thioredoxin sandwich ELISA as part of a study looking at thioredoxin levels in placental trophoblast cells (Di Trapani et al. 1998), a study which also used the antibody for immunoprecipitation of thioredoxin as well as detection of thioredoxin by western blotting. Mouse anti Human thioredoxin antibody, clone 2B1 is also useful for the demonstration of thioredoxin expression on formalin fixed, paraffin embedded material by imunohistochemistry (Lincoln et al. 2003). Clone 2B1 has also been used as a capture reagent in a sandwich ELISA for thioredoxin (Perking et al. 1995)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
9,452 Da
NCBI Official Full Name
thioredoxin isoform 2
NCBI Official Synonym Full Names
thioredoxin
NCBI Official Symbol
TXN
NCBI Official Synonym Symbols
TRX; TRDX; TRX1
NCBI Protein Information
thioredoxin; ADF; ATL-derived factor; SASP; TXN delta 3; surface-associated sulphydryl protein; thioredoxin delta 3
UniProt Protein Name
Thioredoxin
Protein Family
UniProt Gene Name
TXN
UniProt Synonym Gene Names
TRDX; TRX; TRX1; Trx; ADF; SASP
UniProt Entry Name
THIO_HUMAN

NCBI Description

The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

TXN: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA- binding activity in a redox-dependent manner. Up-regulated by ionizing radiation. Belongs to the thioredoxin family.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 9q31

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; protein disulfide oxidoreductase activity; peptide disulfide oxidoreductase activity

Biological Process: negative regulation of protein export from nucleus; transcription, DNA-dependent; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; activation of protein kinase B; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of peptidyl-serine phosphorylation; glycerol ether metabolic process; positive regulation of protein kinase B signaling cascade; cell proliferation; response to radiation; response to reactive oxygen species; cell-cell signaling; cell redox homeostasis; innate immune response; regulation of protein import into nucleus, translocation; cell motility; positive regulation of DNA binding

Research Articles on TXN

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Product Notes

The TXN txn (Catalog #AAA212191) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN THIOREDOXIN reacts with Marmoset and may cross-react with other species as described in the data sheet. AAA Biotech's THIOREDOXIN can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Immunohistology Paraffin, Western Blot (WB). Western Blotting: Minimum Dilution: 10ug/ml; Maximum Dilution: 50ug/ml. Researchers should empirically determine the suitability of the TXN txn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "THIOREDOXIN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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