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Western Blot (WB) (TGFB1I1 monoclonal antibody, Western Blot analysis of TGFB1I1 expression in Hela.)

Mouse anti-Human TGFB1I1 Monoclonal Antibody | anti-TGFB1I1 antibody

TGFB1I1 (ARA55, Transforming Growth Factor beta-1-induced Transcript 1 Protein, Androgen Receptor Coactivator 55kD Protein, Androgen Receptor-associated Protein of 55kD, Hydrogen Peroxide-inducible Clone 5 Protein) (FITC)

Gene Names
TGFB1I1; HIC5; ARA55; HIC-5; TSC-5
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
TGFB1I1; Monoclonal Antibody; TGFB1I1 (ARA55; Transforming Growth Factor beta-1-induced Transcript 1 Protein; Androgen Receptor Coactivator 55kD Protein; Androgen Receptor-associated Protein of 55kD; Hydrogen Peroxide-inducible Clone 5 Protein) (FITC); anti-TGFB1I1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4B2-D8
Specificity
Recognizes human TGFB1I1.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Sequence Length
1790
Applicable Applications for anti-TGFB1I1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-445 from human TGFB1I1 (AAH32545) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MPRSGAPKERPAEPLTPPPSYGHQPQTGSGESSGASGDKDHLYSTVCKPRSPKPAAPAAPPFSSSSGVLGTGLCELDRLLQELNATQFNITDEIMSQFPSSKVASGEQKEDQSEDKKRPSLPSSPSPGLPKASATSATLELDRLMASLSDFRVQNHLPASGPTQPPVVSSTNEGSPSPPEPTGKGSLDTMLGLLQSDLSRRGVPTQAKGLCGSCNKPIAGQVVTALGRAWHPEHFVCGGCSTALGGSSFFEKDGA
Conjugate
FITC
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TGFB1I1 monoclonal antibody, Western Blot analysis of TGFB1I1 expression in Hela.)

Western Blot (WB) (TGFB1I1 monoclonal antibody, Western Blot analysis of TGFB1I1 expression in Hela.)

Western Blot (WB)

(Western Blot analysis of TGFB1I1 expression in transfected 293T cell line by TGFB1I1 monoclonal antibody. Lane 1: TGFB1I1 transfected lysate (47.9kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of TGFB1I1 expression in transfected 293T cell line by TGFB1I1 monoclonal antibody. Lane 1: TGFB1I1 transfected lysate (47.9kD). Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged TGFB1I1 is ~0.03ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged TGFB1I1 is ~0.03ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of TGFB1I1 over-expressed 293 cell line, cotransfected with TGFB1I1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TGFB1I1 monoclonal antibody, GAPDH (36.1kD) used as specificity and loading control.)

Western Blot (WB) (Western blot analysis of TGFB1I1 over-expressed 293 cell line, cotransfected with TGFB1I1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TGFB1I1 monoclonal antibody, GAPDH (36.1kD) used as specificity and loading control.)
Related Product Information for anti-TGFB1I1 antibody
This gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-TGFB1I1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Official Full Name
Homo sapiens transforming growth factor beta 1 induced transcript 1, mRNA
NCBI Official Synonym Full Names
transforming growth factor beta 1 induced transcript 1
NCBI Official Symbol
TGFB1I1
NCBI Official Synonym Symbols
HIC5; ARA55; HIC-5; TSC-5
NCBI Protein Information
transforming growth factor beta-1-induced transcript 1 protein

NCBI Description

This gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Research Articles on TGFB1I1

Similar Products

Product Notes

The TGFB1I1 (Catalog #AAA6150093) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TGFB1I1 (ARA55, Transforming Growth Factor beta-1-induced Transcript 1 Protein, Androgen Receptor Coactivator 55kD Protein, Androgen Receptor-associated Protein of 55kD, Hydrogen Peroxide-inducible Clone 5 Protein) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TGFB1I1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TGFB1I1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGFB1I1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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