Immunohistochemistry (IHC)
(Immunohistochemistry using formalin-fixed, paraffin-embedded human Testicular Carcinoma Stained with 168867-AF)
Mouse TGF-alpha Monoclonal Antibody | anti-TGF-alpha antibody
TGF-alpha (EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1) (BSA & Azide Free) (MaxLight 650)
Reactivity
Bovine, Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
TGF-alpha; Monoclonal Antibody; TGF-alpha (EGF-like TGF; ETGF; TFGA; TGF Type 1; TGFA; Wa1; Waved 1) (BSA & Azide Free) (MaxLight 650); EGF-like TGF; Waved 1; anti-TGF-alpha antibody
Host
Mouse
Reactivity
Bovine, Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
MF9
Specificity
Recognizes human TGF-alpha. Species Crossreactivity: bovine, rat and mouse
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Applicable Applications for anti-TGF-alpha antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
Application Notes
IHC-P: 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponfing to 17aafrom the carboxyl terminus of rat TGF-alpha. Cellular Localization: Cytoplasmic and Extracellular (secreted)
Conjugate
MaxLight650
Positive Control
Jurkat or Ramos cells. Testicular carcinoma
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunohistochemistry (IHC)
(Immunohistochemistry using formalin-fixed, paraffin-embedded human Testicular Carcinoma Stained with 168867-AF)
Immunohistochemistry (IHC)
(Immunohistochemistry using formalin-fixed, paraffin-embedded human Testicular Carcinoma Stained with 168867-AF)
Product Categories/Family for anti-TGF-alpha antibody
References
1. Kobrin MS et. al. J Bio Chem, 1986, 261:14414-9. 2. Kudlow JE et. al. Endocrinology, 1987, 121(4):1577-9. 3. Kobrin MS et. al. Endocrinology, 1987, 121(4):1412-6. 4. Kudlow JE et. al. J Bio Chem, 1989, 264(7):3880-3
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Product Notes
The TGF-alpha (Catalog #AAA6225879) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TGF-alpha (EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1) (BSA & Azide Free) (MaxLight 650) reacts with Bovine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TGF-alpha can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. IHC-P: 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TGF-alpha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGF-alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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