Rabbit anti-Mouse TFPI  Monoclonal Antibody | anti-TFPI  antibody

Rabbit anti Mouse TFPI  Monoclonal Antibody

Cat. Num. AAA2544489

• Gene Names: TFPI; EPI; TFI; LACI; TFPI1
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: TFPI ; Monoclonal Antibody; Rabbit anti Mouse TFPI Monoclonal Antibody; anti-TFPI antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 304

• Applicable Applications for anti-TFPI antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Mouse TFPI. The detection limit for Mouse TFPI is approximately 0.00245 ng/well.
• Immunogen: Recombinant Mouse TFPI Protein
• Buffer: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse TFPI (rM TFPI; NP_035706.1; Met 1-Lys 289).
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -70 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-TFPI  antibody

Tissue factor pathway inhibitor (TFPI) is the natural inhibitor of TF coagulant and signaling activities. It is a Kunitz-type serine proteinase inhibitor that down-regulates tissue factor-initiated blood coagulation. With its Kunitz domains, TFPI exhibits significant homology with human inter-alpha-trypson inhibitor and bovin basic pancreatic trypsin inhibitor. TFPI is the natural inhibitor of TF coagulant and signaling activities. The importance of TFPI in the regulation of blood coagulation is emphasized by how its activity is modulated in human disease. In a factor (F) Xa-dependent feedback system, TFPI binds directly and inhibits the TF-FVII/FVIIa complex. Normally, TFPI exists in plasma both as a full-length molecule and as variably carboxy-terminal truncated forms. TFPI also circulates in complex with plasma lipoproteins. The levels and the dual inhibitor effect of TFPI on FXa and TF-FVII/FVIIa complex offers insight into the mechanisms of various pathological conditions triggered by TF. TFPI may play an important role in modulating TF-induced thrombogenesis and it may also provide a unique therapeutic approach for prophylaxis and/or treatment of various diseases. In addition, Studies have shown that TFPI exhibits antiangiogenic and antimetastatic effects in vitro and in vivo. In animal models of experimental metastasis, both circulating and tumor cell-associated TFPI are shown to significantly reduce tumor cell-induced coagulation activation and lung metastasis.

NCBI and Uniprot Product Information

• NCBI GI #: 1042779777
• NCBI GeneID: 7035
• NCBI GenBank Nucleotide #: NM_001329239.1
• Molecular Weight: 28,653 Da
• NCBI Official Full Name: tissue factor pathway inhibitor isoform a
• NCBI Official Synonym Full Names: tissue factor pathway inhibitor
• NCBI Official Symbol: TFPI
• NCBI Official Synonym Symbols: EPI; TFI; LACI; TFPI1
• NCBI Protein Information: tissue factor pathway inhibitor
• UniProt Protein Name: Tissue factor pathway inhibitor
• UniProt Gene Name: TFPI
• UniProt Synonym Gene Names: LACI; TFPI1; TFPI; EPI; LACI
• UniProt Entry Name: TFPI1_HUMAN

NCBI Description

This gene encodes a Kunitz-type serine protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. Inhibition of the encoded protein restores hemostasis in animal models of hemophilia. This gene encodes multiple protein isoforms that differ in their inhibitory activity, specificity and cellular localization. [provided by RefSeq, Jul 2016]

Uniprot Description

TFPI: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Membrane protein, GPI anchor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: cell surface; extracellular region; extracellular space; plasma membrane

Molecular Function: endopeptidase inhibitor activity

Biological Process: blood coagulation; blood coagulation, extrinsic pathway