Mouse anti-Human TFEB Monoclonal Antibody | anti-TFEB antibody

Anti-TFEB Antibody

Cat. Num. AAA415007

• Gene Names: TFEB; TCFEB; BHLHE35; ALPHATFEB
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity purified
• Synonyms: TFEB; Monoclonal Antibody; Anti-TFEB Antibody; anti-TFEB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Anti-TFEB recognizes TFEB expression in Hela whole cell lysate
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 2364

• Applicable Applications for anti-TFEB antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:1000 analysis of TFEB expression in Hela whole cell lysate with Anti-TFEB
• Raised In: Purified mouse monoclonal antibody generated using E.coli expressed recombinant protein TFEB as immunogen.
• Buffer: citrate-tris-HCl buffer pH7.0 with 0.02% Proclin 300.
• Antibody Type: Primary antibody.
• Immunogen: Recombinant protein corresponding to full length TFEB.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

References

None

NCBI and Uniprot Product Information

• NCBI GI #: 268607698
• NCBI GeneID: 7942
• NCBI Accession #: NP_009093.1
• NCBI GenBank Nucleotide #: NM_007162
• Molecular Weight: 43,803 Da
• NCBI Official Full Name: Homo sapiens transcription factor EB (TFEB), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: transcription factor EB
• NCBI Official Symbol: TFEB
• NCBI Official Synonym Symbols: TCFEB; BHLHE35; ALPHATFEB
• NCBI Protein Information: transcription factor EB; T-cell transcription factor EB; class E basic helix-loop-helix protein 35
• UniProt Protein Name: Transcription factor EB
• Protein Family: Transcription factor
• UniProt Gene Name: TFEB
• UniProt Entry Name: TFEB_HUMAN

Uniprot Description

TFEB: Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Homodimer and heterodimer; with TFE3 or MITF. Belongs to the MiT/TFE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; transcription factor activity

Biological Process: embryonic placenta development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; lysosome organization and biogenesis; autophagy; positive regulation of transcription from RNA polymerase II promoter; humoral immune response; positive regulation of autophagy