Mouse anti-Human TEM8 Monoclonal Antibody | anti-ANTXR1 antibody
TEM8 (Tumor Endothelial Marker 8, Bacillus anthracis (Anthrax) Toxin Receptor, ATR1)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt normal cellular processes. Three alternatively spliced variants that encode different protein isoforms have been described. [provided by RefSeq]
Uniprot Description
ANTXR1: Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells. Defects in ANTXR1 are associated with susceptibility to hemangioma capillary infantile (HCI). HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. Belongs to the ATR family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Actin-binding; Receptor, misc.
Chromosomal Location of Human Ortholog: 2p13.1
Cellular Component: filopodium membrane; cell surface; integral to membrane; plasma membrane; endosome membrane
Molecular Function: collagen binding; actin filament binding; protein binding; transmembrane receptor activity; metal ion binding
Biological Process: actin cytoskeleton reorganization; reproductive process; pathogenesis; signal transduction
Disease: Gapo Syndrome; Hemangioma, Capillary Infantile
Research Articles on ANTXR1
2. Observational study of gene-disease association. (HuGE Navigator)
3. TEM8 expression levels in DC-based therapeutic vaccines would allow the selection of a subgroup of patients who are most likely to benefit from therapeutic vaccination.
4. ANTXR1 does not use an adaptor to bind the cytoskeleton. This peptide orders actin filaments into arrays, demonstrating an actin bundling activity that is novel for a membrane protein
5. This is the first demonstration that the ATR/TEM8 protein is highly expressed in epithelial cells, suggesting that the ATR/TEM8 expression pattern is highly relevant for understanding the pathogenesis of anthrax infection.
6. Data show that cells expressing palmitoylation-defective mutant receptors are less sensitive to anthrax toxin due to a lower number of surface receptors as well as premature internalization of protective antigen without a requirement for heptamerization.
7. Here we describe the cloning of the human PA receptor using a genetic complementation approach
8. results indicate that TEM8 plays a positive role in endothelial cell activities related to angiogenesis
9. because protective antigen binds to CMG2 with much higher affinity than it does to TEM8, a lower pH is needed to attenuate CMG2 binding to allow pore formation; toxin can form pores at different points in the endocytic pathway
10. TEM8 is a new adhesion molecule linking collagen I or PA to the actin cytoskeleton
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Product Notes
The ANTXR1 antxr1 (Catalog #AAA603164) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TEM8 (Tumor Endothelial Marker 8, Bacillus anthracis (Anthrax) Toxin Receptor, ATR1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TEM8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Western Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect human TEM8. Using a colorimetric detection system, the detection limit for rhTEM8 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human TEM8. The detection limit for rhTEM8 is approximately 5 ng/well., Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the ANTXR1 antxr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TEM8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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