Human anthrax toxin receptor 1 ELISA Kit | ANTXR1 elisa kit

Human anthrax toxin receptor 1, ANTXR1 ELISA Kit

Cat. Num. AAA941904

• Gene Names: ANTXR1; ATR; GAPO; TEM8
• Reactivity: Human
• Synonyms: anthrax toxin receptor 1; Human anthrax toxin receptor 1; ANTXR1 ELISA Kit; Human anthrax toxin receptor 1 (ANTXR1) ELISA kit; ATR; FLJ10601; FLJ11298; FLJ21776; TEM8; 2310008J16Rik; 2810405N18Rik; tumor endothelial marker 8; ANTXR1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human ANTXR1. No significant cross-reactivity or interference between human ANTXR1 and analogues was observed.
• Sequence Length: 333

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5 pg/ml-4000 pg/ml
• Sensitivity: < 15.6 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANTXR1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANTXR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANTXR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANTXR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANTXR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 16933553
• NCBI GeneID: 84168
• NCBI Accession #: NP_060623.2
• NCBI GenBank Nucleotide #: NM_018153.3
• UniProt Accession #: Q9H6X2; Q4ZFV6; Q53QD8; Q96P02; Q9NVP3; A8K7U8; J7K7G4; J7KF88
• Molecular Weight: 62,789 Da
• NCBI Official Full Name: anthrax toxin receptor 1 isoform 3
• NCBI Official Synonym Full Names: anthrax toxin receptor 1
• NCBI Official Symbol: ANTXR1
• NCBI Official Synonym Symbols: ATR; GAPO; TEM8
• NCBI Protein Information: anthrax toxin receptor 1; 2310008J16Rik; 2810405N18Rik; tumor endothelial marker 8
• UniProt Protein Name: Anthrax toxin receptor 1
• Protein Family: Anthrax toxin receptor
• UniProt Gene Name: ANTXR1
• UniProt Synonym Gene Names: ATR; TEM8
• UniProt Entry Name: ANTR1_HUMAN

NCBI Description

This gene encodes a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt normal cellular processes. Three alternatively spliced variants that encode different protein isoforms have been described. [provided by RefSeq, Oct 2008]

Uniprot Description

ANTXR1: Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells. Defects in ANTXR1 are associated with susceptibility to hemangioma capillary infantile (HCI). HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. Belongs to the ATR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Actin-binding; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: filopodium membrane; cell surface; plasma membrane; integral to membrane; endosome membrane

Molecular Function: collagen binding; actin filament binding; protein binding; transmembrane receptor activity; metal ion binding

Biological Process: actin cytoskeleton reorganization; reproductive process; pathogenesis; signal transduction

Disease: Gapo Syndrome; Hemangioma, Capillary Infantile