Flow Cytometry (FC/FACS)
(Staining of F9 embryonal carcinoma cell line with 0.25 ug of Rat IgG2a kappa Isotype Control (PE) (open histogram) or 0.25 ug of OCT3/4 antibody (PE) (filled histogram). Staining was carried out using the Foxp3 Staining Buffer Set (00-552). Total cells were used for analysis.)
Rat OCT3/4 Monoclonal Antibody | anti-OCT3/4 antibody
OCT3/4 antibody (PE)
Applications
Flow Cytometry, Functional Assay
Synonyms
OCT3/4; Monoclonal Antibody; OCT3/4 antibody (PE); Monoclonal OCT3/4; Anti-OCT3/4; transcription factor 1; OCT-3/4; Oct-3; POU5F1; POU domain; OCT 3/4; OCT3; Oct-4; class 5; octamer-binding transcription factor; OCT4; anti-OCT3/4 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
EM92
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
200 ug/ml (varies by lot)
Applicable Applications for anti-OCT3/4 antibody
Flow Cytometry (FC/FACS)
Application Notes
This can be used at the same concentration as the experimental antibody
Biological Significance
The EM92 monoclonal antibody reacts with mouse and human Oct-3/4, encoded by the Pou5F1 gene. Oct-3/4 is a POU domain-containing transcription factor that is critical for maintaining embryonic stem cells in a pluripotent state, and is expressed by ES, embryonic germ cells and embryonic carcinoma cell lines. In cells of the inner cell mass, reduction of Oct-3/4 expression causes dedifferentiation to trophoectoderm, whereas increased expression results in differentiation to mesoderm and primitive endoderm. Oct-3/4 regulates the expression of several genes, including FGF-4, UTF1, Sox2, Fbx15, Rex1 and osteopontin through distinct mechanisms. Furthermore, Oct-3/4 frequently acts synergistically with Sox2 to regulate target gene expression, as is the case with FGF-4. It has been demonstrated that Oct-3/4 expression in ES cells can be negatively regulated by either treatment with retinoic acid, or by removal of leukemia-inhibitory factor.
Tag/Conjugate
PE
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of F9 embryonal carcinoma cell line with 0.25 ug of Rat IgG2a kappa Isotype Control (PE) (open histogram) or 0.25 ug of OCT3/4 antibody (PE) (filled histogram). Staining was carried out using the Foxp3 Staining Buffer Set (00-552). Total cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of F9 embryonal carcinoma cell line with 0.25 ug of Rat IgG2a kappa Isotype Control (PE) (open histogram) or 0.25 ug of OCT3/4 antibody (PE) (filled histogram). Staining was carried out using the Foxp3 Staining Buffer Set (00-552). Total cells were used for analysis.)
Related Product Information for anti-OCT3/4 antibody
Rat monoclonal OCT3/4 antibody (PE)
Product Categories/Family for anti-OCT3/4 antibody
Similar Products
Product Notes
The OCT3/4 (Catalog #AAA834281) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's OCT3/4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). This can be used at the same concentration as the experimental antibody. Researchers should empirically determine the suitability of the OCT3/4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OCT3/4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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