Mouse anti-Chicken TCR alpha beta (V beta 1) Monoclonal Antibody
Mouse Anti-Chicken TCRab/Vb1-BIOT
Reactivity
Chicken
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Synonyms
TCR alpha beta (V beta 1); Monoclonal Antibody; Mouse Anti-Chicken TCRab/Vb1-BIOT; Mouse Anti-Chicken TCRab/Vb1 - Biotin; Mouse Anti-Chicken TCR alpha beta (V beta 1); anti-TCR alpha beta (V beta 1) antibody
Host
Mouse
Reactivity
Chicken
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
TCR2
Specificity
Chicken TCR alpha beta (V beta 1)
MAb TCR2 precipitates a CD3-associated heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and 50- kDa upon reduction) on chicken peripheral blood T cells.1 Deglycosylation of the heterodimer yields two polypeptides of Mr 34-kDa and 29-kDa from TCR2 precipitates. In the chicken, two distinct subpopulations of alpha beta T cells appear in the thymus subsequent to the appearance of gamma delta T cells. These subpopulations, originally denoted as TCR2 and TCR3,1-3 arise sequentially in the thymus during ontogeny and are now known to represent two distinct V beta families, V beta 1 and V beta 2, respectively.4 The TCR2 MAb reacts with approximately 40% of thymocytes, 40-50% of blood mononuclear cells and 40% of splenocytes in the chicken. Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74+/-2%) or CD8 (26+/-4%). TCR2+ cells in the spleen also express either CD4 (37+/-1%) or CD8 (64+/-4%). Surprisingly, a relatively large subpopulation of CD8+ cells in the spleen are negative for TCR2.1-3 This observation led to the demonstration that 71+/-6% of the TCR1(gamma delta)+ cells in the spleen express the CD8 marker.1-4
MAb TCR2 precipitates a CD3-associated heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and 50- kDa upon reduction) on chicken peripheral blood T cells.1 Deglycosylation of the heterodimer yields two polypeptides of Mr 34-kDa and 29-kDa from TCR2 precipitates. In the chicken, two distinct subpopulations of alpha beta T cells appear in the thymus subsequent to the appearance of gamma delta T cells. These subpopulations, originally denoted as TCR2 and TCR3,1-3 arise sequentially in the thymus during ontogeny and are now known to represent two distinct V beta families, V beta 1 and V beta 2, respectively.4 The TCR2 MAb reacts with approximately 40% of thymocytes, 40-50% of blood mononuclear cells and 40% of splenocytes in the chicken. Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74+/-2%) or CD8 (26+/-4%). TCR2+ cells in the spleen also express either CD4 (37+/-1%) or CD8 (64+/-4%). Surprisingly, a relatively large subpopulation of CD8+ cells in the spleen are negative for TCR2.1-3 This observation led to the demonstration that 71+/-6% of the TCR1(gamma delta)+ cells in the spleen express the CD8 marker.1-4
Form/Format
Biotin (BIOT) Conjugate
Applicable Applications for anti-TCR alpha beta (V beta 1) antibody
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
Application Notes
Research Application(s): In ovo suppression of the development of TCR-V beta 1 cells
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform with characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScanTM flow cytometer.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Chen, C.H., J. Cihak, U. Losch, and M.D. Cooper. 1988. Eur. J. Immunol. 18:539.
2. Chen, C.H., J.T. Sowder, J.M. Lahti, J. Cihak, U. Losch, and M.D. Cooper. 1989. Proc. Natl. Acad. Sci. (USA):86:2351
3. Char, D., P. Sanchez, C.H. Chen, R.P. Bucy, and M.D. Cooper. 1990. J. Immunol. 145:3547.
4. Chen, C.H., T.W.F. Gobel, T. Kubota, and M.D. Cooper. 1993. 1994. Poultry Science 73:1012.
5. Sowder, J.T., C.H. Chen, L.L. Ager, J. Cihak, U. Losch, and M.D. Cooper. 1988b. Fed. Proc. 2:874.
6. Cihak, J., U. Losch, G. Hoffman-Hezer, C.H. CHen, M.D. Cooper, and H.W.L. Ziegler-Heitbrock. 1993. Scand. J. Immunol. 38:123.
7. Chen, C.H. 1996. Personal communication.
2. Chen, C.H., J.T. Sowder, J.M. Lahti, J. Cihak, U. Losch, and M.D. Cooper. 1989. Proc. Natl. Acad. Sci. (USA):86:2351
3. Char, D., P. Sanchez, C.H. Chen, R.P. Bucy, and M.D. Cooper. 1990. J. Immunol. 145:3547.
4. Chen, C.H., T.W.F. Gobel, T. Kubota, and M.D. Cooper. 1993. 1994. Poultry Science 73:1012.
5. Sowder, J.T., C.H. Chen, L.L. Ager, J. Cihak, U. Losch, and M.D. Cooper. 1988b. Fed. Proc. 2:874.
6. Cihak, J., U. Losch, G. Hoffman-Hezer, C.H. CHen, M.D. Cooper, and H.W.L. Ziegler-Heitbrock. 1993. Scand. J. Immunol. 38:123.
7. Chen, C.H. 1996. Personal communication.
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Product Notes
The TCR alpha beta (V beta 1) (Catalog #AAA673881) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Chicken TCRab/Vb1-BIOT reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's TCR alpha beta (V beta 1) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation. Research Application(s): In ovo suppression of the development of TCR-V beta 1 cells Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells. Researchers should empirically determine the suitability of the TCR alpha beta (V beta 1) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCR alpha beta (V beta 1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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