Mouse anti-Human TCF1 Monoclonal Antibody | anti-TCF1 antibody

TCF1 (T-cell Factor 1) (MaxLight 550)

Cat. Num. AAA6246099

• Gene Names: TCF7; TCF-1
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: TCF1; Monoclonal Antibody; TCF1 (T-cell Factor 1) (MaxLight 550); anti-TCF1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4i63
• Specificity: Recognizes multiple splice isoforms of TcF 1, Mr of 26kD, 32kD, 38kD, 42kD and 48kD. Species Crossreactivity: Does not recognize mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-TCF1 antibody: FLISA, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB)
• Immunogen: Maltose binding protein (MBP) fusion protein corresponding to residues 102-269 of human TcF 1A splice variant. The epitope maps to a region just N-terminal to the High Mobility Group (HMG) box.
• Conjugate: MaxLight550
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Western Blot (WB)

(WB: Jurkat cell lysate probed with anti-TCF-1, T2020,at 1ug/ml)

Product Categories/Family for anti-TCF1 antibody

Antibodies; Transcription Factors

References

1. Castrop, J., et al., Blood 86: 3050-3059, 1995. 2. Van De Wetering, M., et al., Mol. Cell. Biol. 16: 745-752, 1996.

NCBI and Uniprot Product Information

• NCBI GI #: 293651582
• NCBI GeneID: 6932
• NCBI Accession #: NP_001128323.2
• NCBI GenBank Nucleotide #: NM_003202
• UniProt Accession #: P36402; Q86WR9; Q9UKI4; B3KSH3
• Molecular Weight: 41,642 Da
• NCBI Official Full Name: transcription factor 7 isoform 3
• NCBI Official Synonym Full Names: transcription factor 7 (T-cell specific, HMG-box)
• NCBI Official Symbol: TCF7
• NCBI Official Synonym Symbols: TCF-1
• NCBI Protein Information: transcription factor 7; T-cell-specific transcription factor 1
• UniProt Protein Name: Transcription factor 7
• UniProt Gene Name: TCF7
• UniProt Synonym Gene Names: TCF1; TCF-7; T-cell factor 1; TCF-1
• UniProt Entry Name: TCF7_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional activator that plays an important role in lymphocyte differentiation. This gene is expressed predominantly in T-cells. The encoded protein can bind an enhancer element and activate the CD3E gene, and it also may repress the CTNNB1 and TCF7L2 genes through a feedback mechanism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

TCF7: Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T- lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1. Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with AES, TLE1, TLE2, TLE3 and TLE4. By TCF7L2 and CTNNB1. Predominantly expressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium. Belongs to the TCF/LEF family. 16 isoforms of the human protein are produced by alternative promoter.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: nucleoplasm; transcription factor complex; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; beta-catenin binding; chromatin binding; transcription factor activity

Biological Process: neural tube development; transcription, DNA-dependent; alpha-beta T cell differentiation; Wnt receptor signaling pathway through beta-catenin; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; embryonic digestive tract morphogenesis; generation of neurons; T cell receptor V(D)J recombination; immune response; brain development; embryonic genitalia morphogenesis; embryonic hindgut morphogenesis