Western Blot (WB)
(The cell lysates of Jurkat (40ug) and A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCB antibody (1:1500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. )
Mouse anti-Human TBCB Monoclonal Antibody | anti-TBCB antibody
TBCB antibody
Gene Names
TBCB; CG22; CKAP1; CKAPI
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
TBCB; Monoclonal Antibody; TBCB antibody; Tubulin folding cofactor B; CG22; CKAP1; CKAPI; anti-TBCB antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
AT1F6
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
244
Applicable Applications for anti-TBCB antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Recommended starting dilution is 1:1500
Antigen Species
Human
Immunogen
Recombinant human TBCB (1-244aa) purified from E. coli
Clone Note
Anti-human TBCB mAb, clone AT1F6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TBCB protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates of Jurkat (40ug) and A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCB antibody (1:1500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. )
Western Blot (WB)
(The cell lysates of Jurkat (40ug) and A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCB antibody (1:1500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. )
Related Product Information for anti-TBCB antibody
Tubulin folding cofactor B, also known as TBCB, belongs to the Microtubules family. Biosynthesis of functional microtubules involve the participation of several chaperones, termed Tubulin folding cofactors A (TBCA), B (TBCB), D (TBCD), E (TBCE) and C (TBCC), that act on folding intermediates downstream of the cytosolic chaperon, alternatively named TCP. TBCB is a 244 amino acid cytoplasmic protein containing one CAP-Gly domain and in widely expressed. TBCB is involved in the regulation of tubulin heterodimer dissociation and may function as a negative regulator of axonal growth.
Product Categories/Family for anti-TBCB antibody
References
Kortazar D., et al. (2007) Exp Cell Res. 313:425-436.; Tian G., et al. (1997) J Cell Biol. 138: 821-832; Fanarraga ML., et al. (2009) Exp cell Res 315:535-541;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,801 Da
NCBI Official Full Name
tubulin-folding cofactor B isoform 1
NCBI Official Synonym Full Names
tubulin folding cofactor B
NCBI Official Symbol
TBCB
NCBI Official Synonym Symbols
CG22; CKAP1; CKAPI
NCBI Protein Information
tubulin-folding cofactor B
UniProt Protein Name
Tubulin-folding cofactor B
UniProt Gene Name
TBCB
UniProt Synonym Gene Names
CG22; CKAP1
Uniprot Description
Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer (PubMed:9265649). Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth ().
Research Articles on TBCB
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Product Notes
The TBCB tbcb (Catalog #AAA200320) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TBCB antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TBCB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Recommended starting dilution is 1:1500. Researchers should empirically determine the suitability of the TBCB tbcb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TBCB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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