Taq Monoclonal Antibody | anti-Taq antibody

Anti-Taq Monoclonal Antibody (clone 8C1)

Cat. Num. AAA430074

• Applications: - Real-time or regular hot start PCR applications;; - PCR diagnostics, genotyping, SNP etc.
• Purity: > 95% by SDS-PAGE
• Synonyms: Taq; Monoclonal Antibody; Anti-Taq Monoclonal Antibody (clone 8C1); Anti-Taq Monoclonal Antibody; anti-Taq antibody

• Clonality: Monoclonal
• Clone Number: 8C1
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: 10 mM Tris-HCl (pH 7.0), 50 mM KCl, 0.1 mM EDTA, 50% Glycerol

• Applicable Applications for anti-Taq antibody: - Real-time or regular hot start PCR applications;; - PCR diagnostics, genotyping, SNP etc.
• Application Notes: Protocol: ; 1. Before making any dilution of anti-Taq antibody and Taq polymerase, add 1 mug anti-Taq monoclonal antibody to 2 mug Taq DNA polymerase (100-10,000U). Actual ratio of anti-taq to taq should be tested a optimization.; 2. Mix gently and incubate at RT for about 10-20 minutes or store at 4 degree C.; 3. Set up the PCR reaction by following the protocol of a regular thermal cycling condition used for the Taq DNA polymerase.
• Reaction Buffer: The anti-Taq monoclonal antibody reaction buffer is the same buffer used for the thermostable DNA polymerase.
• Associated Activities: No conversion to the covalently closed circular DNA to the nicked or linear form was observed after incubation of 1 ug of pUC19 with antibodies in final concentration of 2.4 ug/ul in 20 ul of reaction mixture containing 25 mM Tris-HCl (pH 7.9), 100 mM NaCl, 10 mM MgCl2 after 16 hours at 37°C.
• Note: For each Taq DNA polymerase or Taq from different batch, the antibody titration test must be performed to find the best ratio of antibody to Taq DNA polymerase. Too much antibody added to Taq DNA polymerase could kill the PCR reaction
• !Protocol: 1. Before making any dilution of anti-Taq antibody and Taq polymerase, add 1 ug anti-Taq monoclonal antibody to 2 ug Taq DNA polymerase (~20 Units).; 2. Mix gently and incubate at RT for about 10-20 minutes or store at 4°C; 3. Set up the PCR reaction by following the protocol of a regular thermal cycling condition used for the Taq DNA polymerase.
• Preparation and Storage: For long-term storage at -20°C. For immediate use, keep at 4°C, stable for up to one month.

Related Product Information for anti-Taq antibody

Anti-Taq monoclonal antibodies were derived from a hybridoma (fusion of mouse myeloma cell and the cells after mouse immunization with Taq DNA Polymerase).
Anti-Taq monoclonal antibody inhibits polymerase activity before the onset of thermal cycling, preventing nonspecific amplification and primer-dimer formation. When the temperature is raised, the antibody is quickly inactivated and PCR proceeds.
Anti-Taq monoclonal antibody is effective with a variety of commercially available Taq DNA polymerases (native or recombinant). The use of hot start anti-Taq monoclonal antibody significantly improves the specificity of PCR amplification what is especially important for PCR-based diagnostics.

Product Categories/Family for anti-Taq antibody

Taq DNA Polymerases