Human Excitatory Amino Acid Transporter 1 (EAAT1) ELISA Kit | EAAT1 elisa kit

Human Excitatory Amino Acid Transporter 1 (EAAT1) ELISA Kit

Cat. Num. AAA2023757

• Gene Names: SLC1A3; EA6; EAAT1; GLAST; GLAST1
• Reactivity: Human
• Synonyms: Excitatory Amino Acid Transporter 1 (EAAT1); Human Excitatory Amino Acid Transporter 1 (EAAT1) ELISA Kit; SLC1A3; EA6; GLAST1; Sodium-dependent glutamate/aspartate transporter 1; Solute Carrier 1 Member 3; Glial High Affinity Glutamate Transporter; GLutamate Aspartate Transporter; EAAT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Excitatory Amino Acid Transporter 1 (EAAT1).; No significant cross-reactivity or interference between Excitatory Amino Acid Transporter 1 (EAAT1) and analogues was observed.
• Sequence Length: 497

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.123ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Excitatory Amino Acid Transporter 1 (EAAT1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Excitatory Amino Acid Transporter 1 (EAAT1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EAAT1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Excitatory Amino Acid Transporter 1 (EAAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Excitatory Amino Acid Transporter 1 (EAAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Excitatory Amino Acid Transporter 1 (EAAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Excitatory Amino Acid Transporter 1 (EAAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for EAAT1 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 584277117
• NCBI GeneID: 6507
• NCBI Accession #: NP_001276869.1
• NCBI GenBank Nucleotide #: NM_001289940.1
• UniProt Accession #: P43003; Q4JCQ8; B2R5T3
• Molecular Weight: 55,149 Da
• NCBI Official Full Name: excitatory amino acid transporter 1 isoform 5
• NCBI Official Synonym Full Names: solute carrier family 1 (glial high affinity glutamate transporter), member 3
• NCBI Official Symbol: SLC1A3
• NCBI Official Synonym Symbols: EA6; EAAT1; GLAST; GLAST1
• NCBI Protein Information: excitatory amino acid transporter 1
• UniProt Protein Name: Excitatory amino acid transporter 1
• UniProt Gene Name: SLC1A3
• UniProt Synonym Gene Names: EAAT1; GLAST; GLAST1; GLAST-1
• UniProt Entry Name: EAA1_HUMAN

NCBI Description

This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]

Uniprot Description

SLC1A3: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Mitochondrial; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: cell projection; cell surface; cell soma; membrane; plasma membrane; fibril; integral to membrane

Molecular Function: glutamate binding; high-affinity glutamate transmembrane transporter activity; sodium:dicarboxylate symporter activity; L-glutamate transmembrane transporter activity

Biological Process: response to drug; response to light stimulus; auditory behavior; cranial nerve development; glutamate biosynthetic process; positive regulation of synaptic transmission; response to antibiotic; synaptic transmission; L-glutamate import; sensory perception of sound; neuron morphogenesis during differentiation; gamma-aminobutyric acid biosynthetic process; response to wounding; neurotransmitter uptake; ion transport; neuromuscular process controlling balance; transmembrane transport

Disease: Episodic Ataxia, Type 6