NCBI Description
The Mediator is a multiprotein coactivator that is required by DNA-binding transcription factors for transcriptional activation of polymerase II (see MIM 180660)-transcribed genes. MED21 is a Mediator subunit predicted to reside in the middle module and appears to be a core subunit found in nearly all Mediator preparations (summary by Sato et al., 2004 [PubMed 15175163]).[supplied by OMIM]
Uniprot Description
MED21: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 21 family.
Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 12p11.23
Cellular Component: Srb-mediator complex
Molecular Function: protein binding; DNA-directed RNA polymerase activity; transcription coactivator activity
Biological Process: regulation of transcription from RNA polymerase II promoter; blastocyst development; transcription, DNA-dependent; stem cell maintenance; positive regulation of transcription from RNA polymerase II promoter
Product Notes
The MED21 med21 (Catalog #
AAA607871) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Suppressor of RNA Polymerase B (SRB7) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Suppressor of RNA Polymerase B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:8000 Western Blot: 1:8000. Researchers should empirically determine the suitability of the MED21 med21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
It is sometimes possible for the material contained within the vial of
"Suppressor of RNA Polymerase B, Monoclonal Antibody" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are
absolutely not suitable for use in any
sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a
product from AAA Biotech, you
are explicitly certifying that said products will be properly tested and used in line with
industry standard. AAA Biotech
and its authorized distribution partners reserve the right to refuse to fulfill any order if
we have any indication that a
purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech
cannot be held
responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any
aspect of this datasheet at
any time and without notice. It is the responsibility of the customer to inform AAA Biotech
of any product performance
issues observed or experienced within 30 days of receipt of said product. To see additional
details on this or any of our
other policies, please see our
Terms & Conditions page.
