Mouse anti-Human SUOX Monoclonal Antibody | anti-SUOX antibody

SUOX (Sulfite Oxidase, Mitochondrial) (Biotin)

Cat. Num. AAA6144677

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SUOX; Monoclonal Antibody; SUOX (Sulfite Oxidase; Mitochondrial) (Biotin); anti-SUOX antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1H1
• Specificity: Recognizes human SUOX.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 2360

• Applicable Applications for anti-SUOX antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-489 from human SUOX (AAH65193) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MGTLLGLGAVLAYQDHRCRAAQESTHIYTKEEVSSHTSPETGIWVTLGSEVFDVTEFVDLHPGGPSKLMLAAGGPLEPFWALYAVHNQSHVRELLAQYKIGELNPEDKVAPTVETSDPYADDPVRHPALKVNSQRPFNAEPPPELLTENYITPNPIFFTRNHLPVPNLDPDTYRLHVVGAPGGQSLSLSLDDLHNFPRYEITVTLQCAGNRRSEMTQVKEVKGLEWRTGAISTARWAGARLCDVLAQAGHQLCETEAHVCFEGLDSDPTGTAYGASIPLARAMDPEAEVLLAYEMNGQPLPRDHGFPVRVVVPGVVGARHVKWLGRVSVQPEESYSHWQRRDYKGFSPSVDWETVDFDSAPSIQELPVQSAITEPRDGETVESGEVTIKGYAWSGGGRAVIRVDVSLDGGLTWQVAKLDGEEQRPRKAWAWRLWQLKAPVPAGQKELNIVCKAVDDGYNVQPDTVAPIWNLRGVLSNAWHRVHVYVSP
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SUOX monoclonal antibody, Western Blot analysis of SUOX expression in HepG2.)

Western Blot (WB)

(Western Blot analysis of SUOX expression in transfected 293T cell line by SUOX monoclonal antibody. Lane 1: SUOX transfected lysate (53.9kD). Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged SUOX is 0.03ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of SUOX over-expressed 293 cell line, cotransfected with SUOX Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SUOX monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Related Product Information for anti-SUOX antibody

Sulfite oxidase is a homodimeric protein localized to the intermembrane space of mitochondria. Each subunit contains a heme domain and a molybdopterin-binding domain. The enzyme catalyzes the oxidation of sulfite to sulfate, the final reaction in the oxidative degradation of the sulfur amino acids cysteine and methionine. Sulfite oxidase deficiency results in neurological abnormalities which are often fatal at an early age. Alternative splicing results in multiple transcript variants encoding identical proteins.

Product Categories/Family for anti-SUOX antibody

Antibodies; Enzymes; Oxidase

NCBI and Uniprot Product Information

• NCBI GI #: 40806987
• NCBI GeneID: 6821
• NCBI Official Full Name: Homo sapiens sulfite oxidase, mRNA
• NCBI Official Synonym Full Names: sulfite oxidase
• NCBI Official Symbol: SUOX
• NCBI Protein Information: sulfite oxidase, mitochondrial
• Protein Family: Sulfite oxidase

NCBI Description

Sulfite oxidase is a homodimeric protein localized to the intermembrane space of mitochondria. Each subunit contains a heme domain and a molybdopterin-binding domain. The enzyme catalyzes the oxidation of sulfite to sulfate, the final reaction in the oxidative degradation of the sulfur amino acids cysteine and methionine. Sulfite oxidase deficiency results in neurological abnormalities which are often fatal at an early age. Alternative splicing results in multiple transcript variants encoding identical proteins. [provided by RefSeq, Jul 2008]