Mouse Sulfotransferase family 1E Monoclonal Antibody | anti-SULT1E1 antibody

Sulfotransferase family 1E, estrogen-preferring, member 1

Cat. Num. AAA4380700

• Gene Names: SULT1E1; EST; STE; EST-1; ST1E1
• Reactivity: Human. Others not tested.
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Sulfotransferase family 1E; Monoclonal Antibody; estrogen-preferring; member 1; EST; EST-1; EST1; Estrogen sulfotransferase; estrone sulfotransferase; ST1E1; STE; Sulfotransferase 1E1; Sulfotransferase; Sulfotransferase estrogen preferring; anti-SULT1E1 antibody

• Host: Mouse
• Reactivity: Human. Others not tested.
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: CPTC-SULT1E1-1
• Specificity: The soluble sulfotransferases contribute to the elimination of xenobiotics, the activation of procarcinogens and the regulation of hormones by catalyzing the sulfate conjugation of these substances. SULT1E1, also known as STE, is a 294 amino acid member of the SULT1 family. Localized to the cytoplasm and expressed in intestine, liver and kidney, SULT1E1 exists as a homodimer that is thought to control estrogen receptor (ER) levels by sulfurylating free estradiol. Defects in the gene encoding SULT1E1 are associated with an increased risk for endometrial cancer, suggesting a role for SULT1E1 in tumorigenesis.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 294

• Applicable Applications for anti-SULT1E1 antibody: Immunohistology (Formalin-Fixed)
• Application Notes: Immunohistology (Formalin-Fixed): 0.5-1ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Immunogen: Recombinant full-length human SULT1E1 protein
• Positive Control: HEP G2, liver, intestine and kidney.
• Cellular Localization: Nuclear
• Chromosome Location: 14q13.3
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Rebbeck, T.R., et al. 2006. J. Natl. Cancer Inst. 98: 1311-1320.; Allali-Hassani, A., et al. 2007. PLoS Biol. 5: e97.

NCBI and Uniprot Product Information

• NCBI GI #: 4885617
• NCBI GeneID: 6783
• NCBI Accession #: NP_005411.1
• NCBI GenBank Nucleotide #: NM_005420.2
• UniProt Accession #: P49888; Q8N6X5
• Molecular Weight: 35kDa
• NCBI Official Full Name: estrogen sulfotransferase
• NCBI Official Synonym Full Names: sulfotransferase family 1E member 1
• NCBI Official Symbol: SULT1E1
• NCBI Official Synonym Symbols: EST; STE; EST-1; ST1E1
• NCBI Protein Information: estrogen sulfotransferase
• UniProt Protein Name: Estrogen sulfotransferase
• Protein Family: Estrogen sulfotransferase
• UniProt Gene Name: SULT1E1
• UniProt Synonym Gene Names: STE; ST1E1

NCBI Description

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that transfers a sulfo moiety to and from estrone, which may control levels of estrogen receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated.