SULT1E1 recombinant protein

SULT1E1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120768

• Gene Names: SULT1E1; EST; STE; EST-1; ST1E1
• Purity: >92% as determined by SDS-PAGE
• Synonyms: SULT1E1; SULT1E1 Protein; Human; Recombinant (His Tag); Human SULT1E1/ST1E1 Protein (His Tag); EST Protein; EST-1 Protein; MGC34459 Protein; ST1E1 Protein; STE Protein; sulfotransferase family 1E member 1; SULT1E1 recombinant protein

• Host: E Coli
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris 0.5M NaCl, 20% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Asn2-Ile294

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human SULT1E1 (NP_005411.1)(Asn 2-Ile 294) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SULT1E1 recombinant protein

Background: Estrogen sulfotransferase, also known as Sulfotransferase, estrogen-preferring, Sulfotransferase 1E1, SULT1E1 and ST1E1, is a cytoplasm enzyme that belongs to the sulfotransferase 1 family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SULT1E1 may control the level of the estrogen receptor by sulfurylating free estradiol. SULT1E1 maximally sulfates beta-estradiol and estrone at concentrations of 2 nM. SULT1E1 also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated. SULT1E1 is a key enzyme in estrogen homeostasis. It plays a central role in the prevention and development of human disease.

References

Bernier F, et al.,1994, J Biol Chem. 269 (45): 28200-5. Strausberg RL, et al.,2003, Proc. Natl. Acad. Sci. 99 (26): 16899-903. Cole,GB. et al., 2010, Proc Natl Acad Sci USA. 107 (14): 6222-7.

NCBI and Uniprot Product Information

• NCBI GI #: 4885617
• NCBI GeneID: 6783
• NCBI Accession #: NP_005411.1
• NCBI GenBank Nucleotide #: NM_005420.2
• UniProt Accession #: P49888; Q8N6X5
• Molecular Weight: 35,126 Da
• NCBI Official Full Name: estrogen sulfotransferase
• NCBI Official Synonym Full Names: sulfotransferase family 1E, estrogen-preferring, member 1
• NCBI Official Symbol: SULT1E1
• NCBI Official Synonym Symbols: EST; STE; EST-1; ST1E1
• NCBI Protein Information: estrogen sulfotransferase; sulfotransferase 1E1; estrone sulfotransferase; sulfotransferase, estrogen-preferring
• UniProt Protein Name: Estrogen sulfotransferase
• Protein Family: Estrogen sulfotransferase
• UniProt Gene Name: SULT1E1
• UniProt Synonym Gene Names: STE; ST1E1
• UniProt Entry Name: ST1E1_HUMAN

NCBI Description

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that transfers a sulfo moiety to and from estrone, which may control levels of estrogen receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

SULT1E1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated. Belongs to the sulfotransferase 1 family.

Protein type: EC 2.8.2.4; Transferase; Energy Metabolism - sulfur; Lipid Metabolism - androgen and estrogen

Chromosomal Location of Human Ortholog: 4q13.1

Cellular Component: cytosol

Molecular Function: steroid sulfotransferase activity; estrone sulfotransferase activity; flavonol 3-sulfotransferase activity; steroid binding

Biological Process: steroid metabolic process; estrogen metabolic process; xenobiotic metabolic process; sulfation; female pregnancy; 3'-phosphoadenosine 5'-phosphosulfate metabolic process