Mouse anti-Human, Mouse STXBP1 / Munc18 / p67 Monoclonal Antibody | anti-STXBP1 antibody

Mouse Monoclonal [clone 6D1] (IgG2a,k) to Human STXBP1 / Munc18 / p67

Cat. Num. AAA246577

• Gene Names: STXBP1; P67; NSEC1; UNC18; RBSEC1; MUNC18-1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: STXBP1 / Munc18 / p67; Monoclonal Antibody; Mouse Monoclonal [clone 6D1] (IgG2a; k) to Human STXBP1 / Munc18 / p67; Anti-STXBP1 / Munc18 / p67 Antibody (clone 6D1) IHC-plus; STXBP1; EIEE4; HUNC18; MUNC18-1; N-Sec1; NSEC1; Protein unc-18 homolog 1; Protein unc-18 homolog A; UNC18; Unc18-1; p67; RBSEC1; Unc-18A; Munc18; Neuronal SEC1; Syntaxin binding protein 1; Syntaxin-binding protein 1; UNC18A; Human STXBP1; anti-STXBP1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 6D1
• Specificity: Human STXBP1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.41 mg/ml (varies by lot)
• Sequence Length: 603

• Applicable Applications for anti-STXBP1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: STXBP1 (NP_003156, 74 a.a. ~ 169 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: STXBP1 / Munc18 / p67 antibody was raised against sTXBP1 (NP_003156, 74 a.a. ~ 169 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-STXBP1 antibody IHC of human brain, neuropil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-STXBP1 antibody IHC of human pancreas, islets of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(STXBP1 monoclonal antibody clone 6D1 Western blot of STXBP1 expression in HeLa.)

Western Blot (WB)

(STXBP1 monoclonal antibody clone 6D1. Western blot of STXBP1 expression in NIH/3T3.)

ELISA (EIA)

(Detection limit for recombinant GST tagged STXBP1 is approximately 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4507297
• NCBI GeneID: 6812
• NCBI Accession #: NP_003156.1
• NCBI GenBank Nucleotide #: NM_003165.3
• UniProt Accession #: P61764; Q28208; Q62759; Q64320; Q96TG8; B1AM97
• Molecular Weight: 68,736 Da
• NCBI Official Full Name: syntaxin-binding protein 1 isoform a
• NCBI Official Synonym Full Names: syntaxin binding protein 1
• NCBI Official Symbol: STXBP1
• NCBI Official Synonym Symbols: P67; NSEC1; UNC18; RBSEC1; MUNC18-1
• NCBI Protein Information: syntaxin-binding protein 1; N-Sec1; unc-18A; unc18-1; neuronal SEC1; protein unc-18 homolog 1; protein unc-18 homolog A
• UniProt Protein Name: Syntaxin-binding protein 1
• Protein Family: Syntaxin-binding protein
• UniProt Gene Name: STXBP1
• UniProt Synonym Gene Names: UNC18A; Unc18-1; Unc-18A
• UniProt Entry Name: STXB1_HUMAN

NCBI Description

This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010]

Uniprot Description

STXBP1: may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. Two splice-variant isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; protein complex; mitochondrion; cytoplasm; plasma membrane; cytosol

Molecular Function: identical protein binding; protein domain specific binding; SNARE binding; syntaxin binding; syntaxin-1 binding; protein N-terminus binding; protein kinase binding

Biological Process: protein stabilization; synaptic vesicle maturation; neurotransmitter secretion; regulation of synaptic vesicle fusion to presynaptic membrane; positive regulation of calcium ion-dependent exocytosis; synaptic transmission; protein transport; platelet degranulation; axon target recognition; glutamate secretion; energy reserve metabolic process; neuromuscular synaptic transmission; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, GABAergic; regulation of insulin secretion; vesicle docking during exocytosis

Disease: Epileptic Encephalopathy, Early Infantile, 4