Western Blot (WB)
(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling STXB2 with M25219 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Mouse anti-Human STXB2 Monoclonal Antibody | anti-STXB2 antibody
STXB2 Mouse mAb
Gene Names
stxB2; L0104; sltIIB; stx2B
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Synonyms
STXB2; Monoclonal Antibody; STXB2 Mouse mAb; FHL5; Hunc18b; MUNC18-2; Protein unc-18 homolog 2; Protein unc-18 homolog B; STXBP2; syntaxin binding protein 2; syntaxin-binding protein 2; SyntaxinBP2; Syntaxin-BP2; Unc18-2; UNC18B; Unc-18B; UNC18Bpp10122; anti-STXB2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Sequence Length
89
Applicable Applications for anti-STXB2 antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200
IP: 10 tests
FC/FACS: 1:100-1:200
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human STXB2.
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Western Blot (WB)
(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling STXB2 with M25219 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Western Blot (WB)
(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling STXB2 with M25219 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Immunoprecipitation (IP)
(STXB2 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25219 of 10 ug. Western blot was performed from the immunoprecipitate using M25219 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)
Immunoprecipitation (IP)
(STXB2 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25219 of 10 ug. Western blot was performed from the immunoprecipitate using M25219 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)
Flow Cytometry (FC/FACS)
(All lanes : STXB2 Mouse mAb at 1/2000 dilutionLane 1 : THP1 cells membrane fractionLane 2 : Jurkat cells membrane fractionLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilutionPredicted band size : 66 KDaObserved band size : 66 KDaBlocking/Dilution buffer : 1x TBST.)
Flow Cytometry (FC/FACS)
(All lanes : STXB2 Mouse mAb at 1/2000 dilutionLane 1 : THP1 cells membrane fractionLane 2 : Jurkat cells membrane fractionLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilutionPredicted band size : 66 KDaObserved band size : 66 KDaBlocking/Dilution buffer : 1x TBST.)
Related Product Information for anti-STXB2 antibody
This gene encodes a member of the STXBP/unc-18/SEC1 family. The encoded protein is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Product Categories/Family for anti-STXB2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted MW: 66.4kDa
NCBI Official Full Name
Shiga toxin 2 subunit B
NCBI Official Symbol
stxB2
NCBI Official Synonym Symbols
L0104; sltIIB; stx2B
NCBI Protein Information
Shiga toxin 2 subunit B
UniProt Protein Name
Shiga-like toxin 2 subunit B
Protein Family
UniProt Gene Name
stxB2
UniProt Synonym Gene Names
stx2B; SLT-2 B subunit; SLT-2b; SLT-IIb; Verotoxin 2 subunit B
Uniprot Description
The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.
Similar Products
Product Notes
The STXB2 stxb2 (Catalog #AAA435133) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The STXB2 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STXB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:5000 IP: 10 tests FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the STXB2 stxb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STXB2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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