Mouse STAT3 Monoclonal Antibody | anti-STAT3 antibody

STAT3, phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF) (Biotin)

Cat. Num. AAA631929

• Gene Names: STAT3; APRF; HIES
• Reactivity: Canine, Human, Mouse
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Purified; Purified by subsequent thilliophillic adsorption and size exclusion chromatography.
• Synonyms: STAT3; Monoclonal Antibody; phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3; Acute-phase Response Factor; APRF) (Biotin); Anti -STAT3; anti-STAT3 antibody

• Host: Mouse
• Reactivity: Canine, Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6D76
• Specificity: Recognizes human STAT3 phosphorylated at Tyr 705. Species Crossreactivity: mouse and canine. Does not crossreact with the non-phosphorylated form of STAT3, nor with unrelated tyrosine-phosphorylated proteins.
• Purity/Purification: Purified; Purified by subsequent thilliophillic adsorption and size exclusion chromatography.
• Form/Format: Supplied as a liquid in PBS, Sucrose, PEG, 0.09% sodium azide. Labeled with Biotin.

• Applicable Applications for anti-STAT3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry and ELISA.; Dilution: Western Blot: 0.5ug/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.; Immunoprecipitation: 1-10ug per 10e6 pervanadate treated HepG2 cells.; Immunocytochemistry: 1-10ug/ml; ELISA: 0.05ug/ml
• Immunogen: Synthetic peptide corresponding to aa703-707 (A703P[pY]LK707) of STAT3 (KLH). Molecular Weight: 92kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) HEPG2 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with MBS631929 (0.5ug/ml) for 1h at RT and developed by ECL (exp. time: 30 sec).)

Related Product Information for anti-STAT3 antibody

The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators invovled in cytokine signaling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes.

Product Categories/Family for anti-STAT3 antibody

Antibodies; Abs to Transcription Factors; STAT

NCBI and Uniprot Product Information

• NCBI GI #: 21618340
• NCBI GeneID: 6774
• NCBI Accession #: NP_644805.1
• NCBI GenBank Nucleotide #: NM_139276.2
• UniProt Accession #: P40763; O14916; Q9BW54; A8K7B8
• Molecular Weight: 88,068 Da
• NCBI Official Full Name: signal transducer and activator of transcription 3 isoform 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 3 (acute-phase response factor)
• NCBI Official Symbol: STAT3
• NCBI Official Synonym Symbols: APRF; HIES
• NCBI Protein Information: signal transducer and activator of transcription 3; DNA-binding protein APRF; acute-phase response factor
• UniProt Protein Name: Signal transducer and activator of transcription 3
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT3
• UniProt Synonym Gene Names: APRF
• UniProt Entry Name: STAT3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; cytoplasm; mitochondrial inner membrane; plasma membrane; nucleus; cytosol

Molecular Function: protein dimerization activity; protein binding; ligand-dependent nuclear receptor activity; signal transducer activity; DNA binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding; protein kinase binding; protein phosphatase binding; CCR5 chemokine receptor binding; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; viral reproduction; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; radial glial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; thermoregulation; signal transduction; glucose homeostasis; response to estradiol stimulus; negative regulation of cell proliferation; astrocyte differentiation; regulation of transcription, DNA-dependent; protein import into nucleus; acute-phase response; negative regulation of glycolysis; positive regulation of Notch signaling pathway; response to drug; nervous system development; intracellular receptor-mediated signaling pathway; eating behavior; cytokine and chemokine mediated signaling pathway; regulation of multicellular organism growth; JAK-STAT cascade; cellular response to hormone stimulus; regulation of transcription from RNA polymerase II promoter; cell proliferation; response to ethanol; sexual reproduction; positive regulation of transcription from RNA polymerase II promoter; eye photoreceptor cell differentiation; cell motility; phosphorylation

Disease: Hyper-ige Recurrent Infection Syndrome, Autosomal Dominant; Autoimmune Disease, Multisystem, Infantile-onset