Immunofluorescence (IF)
(Immunofluorescence staining of human ES cell colony withMBS600089. As anticipated, ES cells within the colony exhibit strong immunoreactivity to this reagent.)
Mouse anti-Human Stage Specific Embryonic Antigen 4 Monoclonal Antibody | anti-SSEA4 antibody
Stage Specific Embryonic Antigen 4 (SSEA4) (HRP)
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
Purified by Protein A Chromatography.
Synonyms
Stage Specific Embryonic Antigen 4; Monoclonal Antibody; Stage Specific Embryonic Antigen 4 (SSEA4) (HRP); anti-SSEA4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG3
Clone Number
5C1
Specificity
Recognizes Stage-specific embryonic antigen 4 (SSEA4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. The differentiation of murine EC. and ES cells may be accompanied by an increase in SSEA-4. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein A Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Applicable Applications for anti-SSEA4 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
Immunogen
Human embryonal carcinoma cell line 2102Ep.
Conjugate
HRP
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Immunofluorescence (IF)
(Immunofluorescence staining of human ES cell colony withMBS600089. As anticipated, ES cells within the colony exhibit strong immunoreactivity to this reagent.)
Immunofluorescence (IF)
(Immunofluorescence staining of human ES cell colony withMBS600089. As anticipated, ES cells within the colony exhibit strong immunoreactivity to this reagent.)
Product Categories/Family for anti-SSEA4 antibody
References
1. Kannagi, R., et al., EMBO Journal 2: 2355-2361 (1983). 2. Fenderson, B.A., et al., Dev. Biol. 122: 21-34 (1987). 3. Shamblott, M.J., et al., PNAS 95: 13,726-13,731 (1998). 4. Thomson, J.A., et al., Science 282: 1145-1147 (1998). 5. Reubinoff, B.E., et al., Nature Biotechnology 18: 399-404 (2000). 6. Henderson, J.K., et al., Stem Cells 20: 329-337 (2002).
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Product Notes
The SSEA4 (Catalog #AAA6244003) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Stage Specific Embryonic Antigen 4 (SSEA4) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Stage Specific Embryonic Antigen 4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the SSEA4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Stage Specific Embryonic Antigen 4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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