Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate All lanes: SA2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 142, 146 kDa Observed band size: 142 kDa)
STAG2 recombinant antibody
STAG2 Antibody
IF: 1:20-1:200
Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate All lanes: SA2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 142, 146 kDa Observed band size: 142 kDa)
Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate All lanes: SA2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 142, 146 kDa Observed band size: 142 kDa)
Immunofluorescence (IF)
(Immunofluorescence staining of MCF7 Cells at 1?50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG ?H+L?.)
Immunofluorescence (IF)
(Immunofluorescence staining of MCF7 Cells at 1?50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG ?H+L?.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a subunit of the cohesin complex, which regulates the separation of sister chromatids during cell division. Targeted inactivation of this gene results in chromatid cohesion defects and aneuploidy, suggesting that genetic disruption of cohesin is a cause of aneuploidy in human cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
Uniprot Description
STAG2: Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1A or SMC1B) and SMC3, which are attached via their hinge domain, and RAD21 which link them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3. Belongs to the SCC3 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: Xq25
Cellular Component: nucleoplasm; intermediate filament cytoskeleton; membrane; plasma membrane; nucleolus; chromosome; chromatin; nucleus; cytosol; chromosome, pericentric region; actin cytoskeleton
Molecular Function: protein binding; chromatin binding
Biological Process: mitosis; meiotic cell cycle; cell division; stem cell maintenance; sister chromatid cohesion; negative regulation of DNA endoreduplication; mitotic cell cycle
Research Articles on STAG2
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Product Notes
The STAG2 stag2 (Catalog #AAA7136638) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STAG2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STAG2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:5000 IF: 1:20-1:200. Researchers should empirically determine the suitability of the STAG2 stag2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAG2, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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