Western Blot (WB)
(Western blot detection of SSX2IP in Jurkat,Hela cell lysates using SSX2IP Rabbit mAb(1:1000 diluted). Predicted band size:71kDa. Observed band size:71kDa.)
Rabbit SSX2IP Monoclonal Antibody | anti-SSX2IP antibody
SSX2IP Rabbit mAb
Gene Names
SSX2IP; ADIP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Affinity purified
Synonyms
SSX2IP; Monoclonal Antibody; SSX2IP Rabbit mAb; ADIP; hMsd1; anti-SSX2IP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Specificity
Widely expressed, with the highest expression in brain, intermediate expression in kidney, testis, spinal cord, liver, heart, lung, skeletal muscle, ovary, fetal liver and fetal brain, and little to no expression in pancreas and spleen. All specific brain regions showed intermediate to high expression, with highest expression in amygdala. Also expressed in fetal tissues, mainly in liver and brain.
Purity/Purification
Affinity purified
Form/Format
50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-SSX2IP antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:2000
IHC: 1:50
ICC/IF: 1:100
IHC: 1:50
ICC/IF: 1:100
Immunogen
A synthetic peptide of human SSX2IP
Conjugation
Unconjugated
Modification
Unmodification
Subcellular Location
Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Nucleus
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot detection of SSX2IP in Jurkat,Hela cell lysates using SSX2IP Rabbit mAb(1:1000 diluted). Predicted band size:71kDa. Observed band size:71kDa.)
Western Blot (WB)
(Western blot detection of SSX2IP in Jurkat,Hela cell lysates using SSX2IP Rabbit mAb(1:1000 diluted). Predicted band size:71kDa. Observed band size:71kDa.)
Related Product Information for anti-SSX2IP antibody
This gene encodes a protein that binds the cancer-testis antigen Synovial Sarcoma X breakpoint 2 protein. The encoded protein may regulate the activity of Synovial Sarcoma X breakpoint 2 protein in malignant cells. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Oct 2009]
Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin. Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling. Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome (PubMed:23816619, PubMed:26545777). Involved in ciliogenesis (PubMed:24356449). It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia (PubMed:24356449).
Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin. Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling. Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome (PubMed:23816619, PubMed:26545777). Involved in ciliogenesis (PubMed:24356449). It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia (PubMed:24356449).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,274 Da
NCBI Official Full Name
afadin- and alpha-actinin-binding protein isoform 1
NCBI Official Synonym Full Names
synovial sarcoma, X breakpoint 2 interacting protein
NCBI Official Symbol
SSX2IP
NCBI Official Synonym Symbols
ADIP
NCBI Protein Information
afadin- and alpha-actinin-binding protein; SSX2-interacting protein; afadin DIL domain-interacting protein
UniProt Protein Name
Afadin- and alpha-actinin-binding protein
Protein Family
UniProt Gene Name
SSX2IP
UniProt Synonym Gene Names
KIAA0923; ADIP
UniProt Entry Name
ADIP_HUMAN
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Product Notes
The SSX2IP ssx2ip (Catalog #AAA4753039) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SSX2IP Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SSX2IP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:2000 IHC: 1:50 ICC/IF: 1:100. Researchers should empirically determine the suitability of the SSX2IP ssx2ip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SSX2IP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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