Mouse anti-Human SRPK1 Monoclonal Antibody | anti-SRPK1 antibody

Anti-SRPK1 Antibody, Mouse Monoclonal

Cat. Num. AAA8106326

• Gene Names: SRPK1; SFRSK1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A
• Synonyms: SRPK1; Monoclonal Antibody; Anti-SRPK1 Antibody; Mouse Monoclonal; SRPK1 Antibody; Mouse MAb; SRSF protein kinase 1; Anti-RP3-422H11.1 Antibody; Anti-SFRSK1 Antibody; anti-SRPK1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 08
• Specificity: Human SRPK1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SRPK1 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:50-1:200
• Immunogen: Recombinant Human SRPK1 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SRPK1 (rh SRPK1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human SRPK1 in human testis with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human SRPK1 in human rectum with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-SRPK1 antibody

Serine/threonine-protein kinase SRPK1, also known as SFRS protein kinase 1, Serine/arginine-rich protein-specific kinase 1, SR-protein-specific kinase 1 and SRPK1, is a cytoplasm and nucleus protein that belongs to the protein kinase superfamily and CMGC Ser/Thr protein kinase family. Isoform 2 of SRPK1 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 of SRPK1 is only seen in the testis, at lower levels than isoform 2. SRPK1 hyperphosphorylates RS domain-containing proteins such as SFRS1, SFRS2 and ZRSR2 on serine residues during metaphase but at lower levels during interphase. SRPK1 plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. SRPK1 locks onto SFRS1 to form a stable complex and processively phosphorylates the RS domain. SRPK1 appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.

References

Ngo J.C.K. et al., 2005, Mol. Cell 20:77-89. Krishnakumar, S. et al., 2008, Pediatr Blood Cancer 50 (2):402-6. Ma, C.T. et al., 2009, J Mol Biol 390 (4):618-34. Souki S.K. et al., 2009, J. Virol. 83:8970-8975. Choudhary C. et al., 2009, Science 325:834-840.

NCBI and Uniprot Product Information

• NCBI GI #: 23468345
• NCBI GeneID: 6732
• Molecular Weight: 72,383 Da
• NCBI Official Full Name: SFRS protein kinase 1
• NCBI Official Synonym Full Names: SRSF protein kinase 1
• NCBI Official Symbol: SRPK1
• NCBI Official Synonym Symbols: SFRSK1
• NCBI Protein Information: SRSF protein kinase 1
• Protein Family: SRSF protein kinase

NCBI Description

This gene encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) family of splicing factors. The protein localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. Alternative splicing of this gene results in multiple transcript variants. Additional alternatively spliced transcript variants have been described for this gene, but their full length nature have not been determined.[provided by RefSeq, Jul 2010]