Western Blot (WB)
(Western blot analysis of Src using 20µg of whole cell lysate (Lane 2=HeLa, Lane 3=3T3, Lane 4=PC12) probed with with Src, mAb (5A18) at 1µg/ml.)
Mouse Src Monoclonal Antibody | anti-Proto-oncogene c-Src; pp60c-src antibody
Src monoclonal antibody (5A18)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~60kDa by Western blot.
Western Blot (WB)
(Western blot analysis of Src using 20µg of whole cell lysate (Lane 2=HeLa, Lane 3=3T3, Lane 4=PC12) probed with with Src, mAb (5A18) at 1µg/ml.)
Western Blot (WB)
(Western blot analysis of Src using 20µg of whole cell lysate (Lane 2=HeLa, Lane 3=3T3, Lane 4=PC12) probed with with Src, mAb (5A18) at 1µg/ml.)
NCBI and Uniprot Product Information
NCBI Description
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Src: proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described.
Protein type: Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Oncoprotein; Protein kinase, TK; TK group; Src family
Chromosomal Location of Human Ortholog: 20q12-q13
Cellular Component: actin filament; caveola; cytoplasm; cytosol; extrinsic to internal side of plasma membrane; late endosome; lysosome; mitochondrial inner membrane; mitochondrion; neuron projection; nucleus; perinuclear region of cytoplasm; plasma membrane; postsynaptic density
Molecular Function: ATP binding; enzyme binding; ephrin receptor binding; estrogen receptor binding; heme binding; hormone receptor binding; insulin receptor binding; integrin binding; kinase activity; kinase binding; non-membrane spanning protein tyrosine kinase activity; phosphoprotein binding; protein binding; protein C-terminus binding; protein kinase activity; protein kinase C binding; protein-tyrosine kinase activity; receptor binding; SH2 domain binding; SH3/SH2 adaptor activity
Biological Process: activation of protein kinase B; axon guidance; blood coagulation; bone resorption; cell adhesion; cell cycle; cell proliferation; cell-cell adhesion; cellular response to insulin stimulus; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; forebrain development; innate immune response; integrin-mediated signaling pathway; leukocyte migration; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of focal adhesion formation; negative regulation of mitochondrial depolarization; negative regulation of protein homooligomerization; negative regulation of telomerase activity; negative regulation of telomere maintenance via telomerase; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; oogenesis; peptidyl-serine phosphorylation; peptidyl-tyrosine phosphorylation; platelet activation; positive regulation of apoptosis; positive regulation of cyclin-dependent protein kinase activity; positive regulation of cytokine secretion; positive regulation of insulin receptor signaling pathway; positive regulation of integrin activation; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein amino acid autophosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of smooth muscle cell migration; positive regulation of transcription, DNA-dependent; progesterone receptor signaling pathway; protein amino acid autophosphorylation; protein destabilization; Ras protein signal transduction; regulation of bone resorption; regulation of cell cycle; regulation of cell proliferation; regulation of cell-cell adhesion; regulation of estrogen receptor signaling pathway; regulation of protein binding; regulation of vascular permeability; response to acidity; response to drug; response to electrical stimulus; response to hydrogen peroxide; response to mechanical stimulus; response to mineralocorticoid stimulus; response to nutrient levels; response to virus; signal complex assembly; signal transduction; small GTPase mediated signal transduction; stimulatory C-type lectin receptor signaling pathway; stress fiber formation; T cell costimulation; transcytosis; transforming growth factor beta receptor signaling pathway; uterus development; vascular endothelial growth factor receptor signaling pathway; viral reproduction
Research Articles on Proto-oncogene c-Src; pp60c-src
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Product Notes
The Proto-oncogene c-Src; pp60c-src src (Catalog #AAA565087) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Src monoclonal antibody (5A18) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Src can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) (PS), Western Blot (WB). Western Blot (1ug/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Detects a band of ~60kDa by Western blot. Researchers should empirically determine the suitability of the Proto-oncogene c-Src; pp60c-src src for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Src, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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