Mouse anti-Human SPRY1 / Sprouty 1 Monoclonal Antibody | anti-SPRY1 antibody

Mouse Monoclonal [clone 3H4] (IgG2a,k) to Human SPRY1 / Sprouty 1

Cat. Num. AAA246050

• Gene Names: SPRY1; hSPRY1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: SPRY1 / Sprouty 1; Monoclonal Antibody; Mouse Monoclonal [clone 3H4] (IgG2a; k) to Human SPRY1 / Sprouty 1; Anti-SPRY1 / Sprouty 1 Antibody (clone 3H4) IHC-plus; SPRY1; HSPRY1; Protein sprouty homolog 1; Spry-1; Sprouty 1; Human SPRY1; anti-SPRY1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3H4
• Specificity: Human SPRY1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4.
• Concentration: 0.49 mg/ml (varies by lot)
• Sequence Length: 319

• Applicable Applications for anti-SPRY1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: SPRY1 (NP_005832, 1 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: SPRY1 / Sprouty 1 antibody was raised against sPRY1 (NP_005832, 1 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-SPRY1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of SPRY1 expression in transfected 293T cell line by SPRY1 monoclonal antibody, clone 3H4.)

Western Blot (WB)

(SPRY1 monoclonal antibody, clone 3H4. Western blot of SPRY1 expression in HepG2.)

ELISA (EIA)

(Detection limit for recombinant GST tagged SPRY1 is approximately 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 384475544
• NCBI GeneID: 10252
• NCBI Accession #: NP_001244967.1
• NCBI GenBank Nucleotide #: NM_001258038.1
• UniProt Accession #: O43609; Q6PNE0; D3DNX6
• Molecular Weight: 35,122 Da
• NCBI Official Full Name: protein sprouty homolog 1
• NCBI Official Synonym Full Names: sprouty homolog 1, antagonist of FGF signaling (Drosophila)
• NCBI Official Symbol: SPRY1
• NCBI Official Synonym Symbols: hSPRY1
• NCBI Protein Information: protein sprouty homolog 1; spry-1; sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling)
• UniProt Protein Name: Protein sprouty homolog 1
• Protein Family: Protein sprouty
• UniProt Gene Name: SPRY1
• UniProt Synonym Gene Names: Spry-1
• UniProt Entry Name: SPY1_HUMAN

Uniprot Description

SPRY1: May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. Belongs to the sprouty family. Induced by FGF signaling.

Protein type: Motility/polarity/chemotaxis; Inhibitor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4q28.1

Cellular Component: plasma membrane; cytosol

Molecular Function: protein binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of cell proliferation; negative regulation of MAP kinase activity; establishment of mitotic spindle orientation; negative regulation of fibroblast growth factor receptor signaling pathway; ureteric bud development; negative regulation of nerve growth factor receptor signaling pathway; induction of an organ; negative regulation of Ras protein signal transduction; metanephros development