Western Blot (WB)
(Figure 1: Western blot analysis using SPP1 mAb against human SPP1 recombinant protein. (Expected MW is 42.6 kDa))
Mouse anti-Human SPP1 Monoclonal Antibody | anti-SPP1 antibody
SPP1 Antibody
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Affinity-Chromatography
Synonyms
SPP1; Monoclonal Antibody; SPP1 Antibody; BNSP; Bone sialoprotein 1; BSP I; BSPI; Early T lymphocyte activation 1; ETA 1; ETA1; MGC110940; Nephropontin; OPN; Osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; OSTP_HUMAN; PSEC0156; secreted phosphoprotein 1 (osteopontin; bone sialoprotein I; early T-lymphocyte activation 1); Secreted phosphoprotein 1; SPP 1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; Uropontin; anti-SPP1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
SPP1 antibody detects endogenous levels of total SPP1
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
300
Applicable Applications for anti-SPP1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
ICC: 1:200-1:1000
WB: 1:500-1:2000
IHC: 1:200-1:1000
ICC: 1:200-1:1000
Immunogen
Purified recombinant fragment of human SPP1 expressed in E. Coli
Subcellular Location
Secreted.
Tissue Specificity
Bone. Found in plasma.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using SPP1 mAb against human SPP1 recombinant protein. (Expected MW is 42.6 kDa))
Western Blot (WB)
(Figure 1: Western blot analysis using SPP1 mAb against human SPP1 recombinant protein. (Expected MW is 42.6 kDa))
Related Product Information for anti-SPP1 antibody
Description: The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
Function: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Subunit Structure: Ligand for integrin alpha-V/beta-3.
Post-translational Modifications: Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. N-and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.
Similarity: Belongs to the osteopontin family.
Function: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Subunit Structure: Ligand for integrin alpha-V/beta-3.
Post-translational Modifications: Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. N-and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.
Similarity: Belongs to the osteopontin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 35.4 kDa
Predicted: 36 kDa
Predicted: 36 kDa
NCBI Official Full Name
osteopontin isoform OPN-b
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
SPP1
NCBI Official Synonym Symbols
OPN; BNSP; BSPI; ETA-1
NCBI Protein Information
osteopontin
UniProt Protein Name
Osteopontin
Protein Family
UniProt Gene Name
SPP1
UniProt Synonym Gene Names
BNSP; OPN; SPP-1
NCBI Description
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Research Articles on SPP1
Similar Products
Product Notes
The SPP1 spp1 (Catalog #AAA9602286) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SPP1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000 IHC: 1:200-1:1000 ICC: 1:200-1:1000. Researchers should empirically determine the suitability of the SPP1 spp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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