Immunocytochemsitry (ICC)
( staining SOX1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit anti-Human, Mouse SOX1 Monoclonal Antibody | anti-SOX1 antibody
SOX1 Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
SOX1; Monoclonal Antibody; SOX1 Antibody; Sex determining region Y box 1 antibody; sox1 antibody;SOX1_HUMAN antibody; SRY antibody; SRY box containing gene 1 antibody; RY related HMG box gene 1 antibody; Transcription factor Sox-1 antibody; anti-SOX1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
JJ20-40
Purity/Purification
ProA affinity purified
Form/Format
Liquid; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL. (varies by lot)
Sequence Length
391
Applicable Applications for anti-SOX1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1,000; ICC: 1:50-1:100; IHC: 1:50-1:100
Immunogen
recombinant protein
Positive control
SHG-44.
Conjugate
Non-conjugated
Subcellular Location
Nucleus.
Preparation and Storage
Store at +4 degree C after thawing. Aliquot store at -20 degree C or -80 degree C. Avoid repeated freeze/thaw cycles.
Immunocytochemsitry (ICC)
( staining SOX1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemsitry (ICC)
( staining SOX1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-SOX1 antibody
Description:
Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.
Background:
Popovic J et al. Expression analysis of SOX14 during retinoic acid induced neural differentiation of embryonal carcinoma cells and assessment of the effect of its ectopic expression on SOXB members in HeLa cells. PLoS One 9:e91852 (2014). Ko HA et al. Cell type-selective expression of the zinc finger-containing gene Nolz-1/Zfp503 in the developing mouse striatum. Neurosci Lett 548:44-9 (2013).
Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.
Background:
Popovic J et al. Expression analysis of SOX14 during retinoic acid induced neural differentiation of embryonal carcinoma cells and assessment of the effect of its ectopic expression on SOXB members in HeLa cells. PLoS One 9:e91852 (2014). Ko HA et al. Cell type-selective expression of the zinc finger-containing gene Nolz-1/Zfp503 in the developing mouse striatum. Neurosci Lett 548:44-9 (2013).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39 kDa
NCBI Official Full Name
transcription factor SOX-1
NCBI Official Synonym Full Names
SRY-box 1
NCBI Official Symbol
SOX1
NCBI Protein Information
transcription factor SOX-1
UniProt Protein Name
Transcription factor SOX-1
Protein Family
UniProt Gene Name
SOX1
NCBI Description
This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. In mice, a similar protein regulates the gamma-crystallin genes and is essential for lens development. [provided by RefSeq, Jul 2008]
Uniprot Description
Transcriptional activator. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation ().
Research Articles on SOX1
Similar Products
Product Notes
The SOX1 sox1 (Catalog #AAA8534243) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SOX1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SOX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:500-1:1,000; ICC: 1:50-1:100; IHC: 1:50-1:100. Researchers should empirically determine the suitability of the SOX1 sox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOX1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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