Mouse anti-Human SNRPA Monoclonal Antibody | anti-SNRPA antibody

SNRPA (U1 Small Nuclear Ribonucleoprotein A, U1 snRNP A, U1-A, U1A) (PE)

Cat. Num. AAA6160411

• Gene Names: SNRPA; U1A; Mud1; U1-A
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SNRPA; Monoclonal Antibody; SNRPA (U1 Small Nuclear Ribonucleoprotein A; U1 snRNP A; U1-A; U1A) (PE); anti-SNRPA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3F9-1F7
• Specificity: Recognizes human SNRPA.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SNRPA antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-283 from human SNRPA (AAH00405) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAVPETRPNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGTFVERDRKREKRKPKSQETPATKKAVQGGGATPVVGAVQGPVPGMPPMTQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQLMPGQMPPAQPLSENPPNHILFLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAGAARDALQGFKITQNNAMKISFAKK
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (57.13kD).)

Western Blot (WB)

(SNRPA monoclonal antibody, Western Blot analysis of SNRPA expression in Hela.)

Western Blot (WB)

(Western Blot analysis of SNRPA expression in transfected 293T cell line by SNRPA monoclonal antibody. Lane 1: SNRPA transfected lysate (31.3kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to SNRPA on formalin-fixed paraffin-embedded human heart tissue. [antibody concentration 3ug/ml].)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to SNRPA on HeLa cell. [antibody concentration 10ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged SNRPA is ~0.03ng/ml as a capture antibody.)

Product Categories/Family for anti-SNRPA antibody

Antibodies; Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 38197143
• NCBI GeneID: 6626
• UniProt Accession #: P09012
• Molecular Weight: 31,280 Da
• NCBI Official Full Name: Homo sapiens small nuclear ribonucleoprotein polypeptide A, mRNA
• NCBI Official Synonym Full Names: small nuclear ribonucleoprotein polypeptide A
• NCBI Official Symbol: SNRPA
• NCBI Official Synonym Symbols: U1A; Mud1; U1-A
• NCBI Protein Information: U1 small nuclear ribonucleoprotein A
• UniProt Protein Name: U1 small nuclear ribonucleoprotein A
• Protein Family: U1 small nuclear ribonucleoprotein
• UniProt Gene Name: SNRPA
• UniProt Synonym Gene Names: U1 snRNP A; U1-A; U1A
• UniProt Entry Name: SNRPA_HUMAN

NCBI Description

The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation. [provided by RefSeq, Oct 2010]

Uniprot Description

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.