Mouse SNF2h Monoclonal Antibody | anti-SMARCA5 antibody

SNF2h (SMARCA5, Sucrose Nonfermenting Protein 2 Homolog, ATP-dependent Chromatin Remodeling Protein, hISWI, hSNF2H, ISWI, Sucrose Nonfermenting-like 5, SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin A5, SWI/SNF Related Matrix Ass

Cat. Num. AAA603486

• Gene Names: SMARCA5; ISWI; SNF2H; hISWI; hSNF2H; WCRF135
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: SNF2h; Monoclonal Antibody; SNF2h (SMARCA5; Sucrose Nonfermenting Protein 2 Homolog; ATP-dependent Chromatin Remodeling Protein; hISWI; hSNF2H; ISWI; Sucrose Nonfermenting-like 5; SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin A5; SWI/SNF Related Matrix Ass; Anti -SNF2h (SMARCA5; anti-SMARCA5 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5i116
• Specificity: Recognizes the small subunit (135kD) of the RSF complex. This complex is capable of facilitating ATP-dependent nucleosome remodeling and transcription initiation from chromatin templates.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.

• Applicable Applications for anti-SMARCA5 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blotting, and Immunoprecipitation.; Dilution: ELISA: 1:8000
• Immunogen: Purified Remodeling and Spacing Factor (RSF).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SMARCA5 antibody

A number of proteins with helicase like motifs are contained within the Snf2p family, named according to the Snf2p/Swi2p protein from Saccharomyces cerevisiae (for review see: Muchardt and Yaniv, 1999) The Snf2p/Swi2p protein has originally been characterized as protein required for sucrose fermentation (SNF = sucrose-non-fermenting) and for switching the mating type locus by the HO endonuclease (SWI = switch). Several proteins similar to Snf2p are members of the Snf/Swi family. According to a phylogenetic analysis of Eisen et al. (1995), the Snf2p family can be divided in subfamilies according to sequence homologies within and particularly outside the core region. Indeed, several of these proteins contain characteristic motifs, such as bromodomains, chromodomains and ring-fingers. The Snf/Swi complex in yeast relies on the Snf2p ATPase activity for its chromatin remodelling activity. The closest homolog of the Snf2p protein is Sth1p. The STH1 gene is, in contrast to SNF2, essential. The Sth1p pprotein is part of the RSC (Remodel the Structure of Chromatin) complex. Many of the Snf/Swi proteins are involved in growth control and cell cycle regulation and there absence or reduced dosage in heterozygote states are associated with cancer (Muchardt & Yaniv, 2001).

Members of the Snf2p family have been shown to be ATPases stimulated by naked DNA (e.g. Snf2p), proteins (e.g. Mot1p; Auble et al. 1997) or nucleosomes (e.g. Isw1p, Isw2p; Tsukiyama et al. 1999). However, no helicase activity has been demonstrated so far for any of these proteins and they may represent ATP-driven motors or translocases involved in structural rearrangements (Havas et al., 2000). Genetic and biochemical characterisations have shown that members of this family are involved in transcription activation, nucleosome rearrangements, recombination, repair and transcription coupled repair (TCR). Indeed, many of these proteins have been shown to be part of multi-subunit complexes. Proteins from Drosophila and mammals have been shown to be required in developmental processes, as it can be expected from the above described activities.

Product Categories/Family for anti-SMARCA5 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 325651836
• NCBI GeneID: 8467
• NCBI Accession #: NP_003592.3
• NCBI GenBank Nucleotide #: NM_003601.3
• UniProt Accession #: O60264
• Molecular Weight: 121,905 Da
• NCBI Official Full Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
• NCBI Official Synonym Full Names: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
• NCBI Official Symbol: SMARCA5
• NCBI Official Synonym Symbols: ISWI; SNF2H; hISWI; hSNF2H; WCRF135
• NCBI Protein Information: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; sucrose nonfermenting-like 5; sucrose nonfermenting protein 2 homolog; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5
• UniProt Protein Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
• Protein Family: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily
• UniProt Gene Name: SMARCA5
• UniProt Synonym Gene Names: SNF2H; WCRF135; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; hSNF2H
• UniProt Entry Name: SMCA5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SMARCA5: an ubiquitous enzyme with ATP-dependent DNA helicase activity. Possesses intrinsic ATP-dependent nucleosome remodelling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin in vitro; this may require intact histone H4 tails. The catalytic subunit of the four known chromatin remodelling complexes: CHRAC, RSF, ACF/WCRF, and WICH. Each complex contains subunits which may regulate the specificity or catalytic activity of SMARCA5. Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy. Belongs to the SNF2/RAD54 helicase family, ISWI subfamily.

Protein type: EC 3.6.4.-; EC 3.6.1.-; Histone-binding; Transcription factor; Helicase

Chromosomal Location of Human Ortholog: 4q31.1-q31.2

Cellular Component: nucleoplasm; chromatin silencing complex; RSF complex; nuclear replication fork; nucleolus; condensed chromosome; nucleus

Molecular Function: protein binding; DNA binding; histone binding; ATPase activity; nucleosome binding; helicase activity; ATP binding

Biological Process: regulation of transcription from RNA polymerase II promoter; chromatin remodeling; chromatin silencing at rDNA; nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; negative regulation of gene expression, epigenetic; double-strand break repair; transcription initiation; ATP-dependent chromatin remodeling; gene expression; regulation of gene expression, epigenetic; nucleosome positioning