Mouse anti-Human SMC3 Monoclonal Antibody | anti-SMC3 antibody

SMC3 (Structural Maintenance of Chromosomes Protein 3, SMC Protein 3, SMC-3, Basement Membrane-associated Chondroitin Proteoglycan, Bamacan, Chondroitin Sulfate Proteoglycan 6, Chromosome-associated Polypeptide, hCAP, BAM, BMH, CSPG6, SMC3L1) (FITC)

Cat. Num. AAA6149779

• Gene Names: SMC3; BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SMC3; Monoclonal Antibody; SMC3 (Structural Maintenance of Chromosomes Protein 3; SMC Protein 3; SMC-3; Basement Membrane-associated Chondroitin Proteoglycan; Bamacan; Chondroitin Sulfate Proteoglycan 6; Chromosome-associated Polypeptide; hCAP; BAM; BMH; CSPG6; SMC3L1) (FITC); anti-SMC3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 2F11
• Specificity: Recognizes human SMC3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-SMC3 antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa711-810 of human SMC3 (BC047324; AAH47324) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: IDQLMNQMQQIETQQRKFKASRDSILSEMKMLKEKRQQSEKTFMPKQRSLQSLEASLHAMESTRESLKAELGTDLLSQLSLEDQKRVDALNDEIRQLQQE
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against immunogen (37kD).)

Western Blot (WB)

(Western Blot analysis of SMC3 expression in transfected 293T cell line using. Lane 1: SMC3 transfected lysate (141.5kD). Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for is ~0.3ng/ml as a capture antibody.)

Related Product Information for anti-SMC3 antibody

This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein.

Product Categories/Family for anti-SMC3 antibody

Antibodies; Membrane Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 28958117
• NCBI GeneID: 9126
• Molecular Weight: 141,542 Da
• NCBI Official Full Name: Homo sapiens structural maintenance of chromosomes 3, mRNA
• NCBI Official Synonym Full Names: structural maintenance of chromosomes 3
• NCBI Official Symbol: SMC3
• NCBI Official Synonym Symbols: BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
• NCBI Protein Information: structural maintenance of chromosomes protein 3

NCBI Description

This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq, Jul 2008]