Mouse Slo2.2 Monoclonal Antibody | anti-Kcnt1 antibody

Slo2.2 Antibody: ATTO 594

Cat. Num. AAA802388

• Gene Names: Kcnt1; Slack; rSlo2
• Reactivity: Human (weak), Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Synonyms: Slo2.2; Monoclonal Antibody; Slo2.2 Antibody: ATTO 594; KCNT1; potassium channel subfamily T member 1; bA100C15.2; SLACK; KCa4.1KIAA1422FLJ41282; anti-Kcnt1 antibody

• Host: Mouse
• Reactivity: Human (weak), Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S3-26
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 1237

• Applicable Applications for anti-Kcnt1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)
• Conjugate: ATTO 594
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone S3-26. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining around nucleus and some in cytoplasm.)

Western Blot (WB)

(Western Blot analysis of Rat brain membrane lysate showing detection of Slo2.2 Potassium Channel protein using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone S3-26. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone S3-26. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Suprabasal epidermal staining. Hair follicles negative.)

Related Product Information for anti-Kcnt1 antibody

Background Info: Detects ~140kDa.

Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Slo2.2 is a novel member of the mammalian Slo potassium channel gene family. Slo2 channels may contribute to the resting potentials of cells that control their basal level of excitability (3). They also have sensors that couple channel activity

Product Categories/Family for anti-Kcnt1 antibody

Ion Channels; Neuroscience

References

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Santi C.M., et al. (2006) J Neuroscience, 26(19): 5059- 5068. 4. Yuan A., et al. (2003) Neuron 37: 765-773.

NCBI and Uniprot Product Information

• NCBI GI #: 11177892
• NCBI GeneID: 60444
• NCBI Accession #: NP_068625
• NCBI GenBank Nucleotide #: NM_021853.1
• UniProt Accession #: Q9Z258; Q5D6C6
• Molecular Weight: 136,827 Da
• NCBI Official Full Name: potassium channel subfamily T member 1
• NCBI Official Synonym Full Names: potassium channel, subfamily T, member 1
• NCBI Official Symbol: Kcnt1
• NCBI Official Synonym Symbols: Slack; rSlo2
• NCBI Protein Information: potassium channel subfamily T member 1; potassium channel subunit (Slack); sequence like a calcium-activated potassium channel subunit
• UniProt Protein Name: Potassium channel subfamily T member 1
• Protein Family: Potassium channel subfamily
• UniProt Gene Name: Kcnt1
• UniProt Synonym Gene Names: Slack
• UniProt Entry Name: KCNT1_RAT

NCBI Description

Na(+)-activated potassium channel; may be involved in regulating the firing properties of neurons [RGD, Feb 2006]

Uniprot Description

KCNT1: Outwardly rectifying potassium channel subunit that may co-assemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro). Belongs to the potassium channel family. Calcium- activated (TC 1.A.1.3) subfamily. KCa4.1/KCNT1 sub-subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, potassium; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: voltage-gated potassium channel complex; membrane; integral to membrane

Molecular Function: calcium-activated potassium channel activity; voltage-gated potassium channel activity; potassium channel activity

Biological Process: potassium ion transport