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Immunohistochemistry (IHC) (Anti-SLC13A5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Mouse anti-Human SLC13A5 Monoclonal Antibody | anti-SLC13A5 antibody

Mouse Monoclonal [clone 2G4] (IgG1,k) to Human SLC13A5

Gene Names
SLC13A5; NACT; mIndy; EIEE25
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Protein A Purified
Synonyms
SLC13A5; Monoclonal Antibody; Mouse Monoclonal [clone 2G4] (IgG1; k) to Human SLC13A5; Anti-SLC13A5 Antibody (clone 2G4) IHC-plus; Na(+)/citrate cotransporter; NACT; Human SLC13A5; anti-SLC13A5 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2G4
Specificity
Human SLC13A5
Purity/Purification
Protein A Purified
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Concentration
0.2 mg/ml (varies by lot)
Sequence Length
522
Applicable Applications for anti-SLC13A5 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.
Target Species
Human
Immunogen Description
SLC13A5 (NP_808218, 152 a.a. ~ 207 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
SLC13A5 antibody was raised against sLC13A5 (NP_808218, 152 a.a. ~ 207 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-SLC13A5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-SLC13A5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(SLC13A5 monoclonal antibody, clone 2G4. Western blot of SLC13A5 expression in HepG2.)

Western Blot (WB) (SLC13A5 monoclonal antibody, clone 2G4. Western blot of SLC13A5 expression in HepG2.)

ELISA (EIA)

(Detection limit for recombinant GST tagged SLC13A5 is 0.03 ng/ml as a capture antibody.)

ELISA (EIA) (Detection limit for recombinant GST tagged SLC13A5 is 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
58,092 Da
NCBI Official Full Name
solute carrier family 13 member 5 isoform b
NCBI Official Synonym Full Names
solute carrier family 13 (sodium-dependent citrate transporter), member 5
NCBI Official Symbol
SLC13A5
NCBI Official Synonym Symbols
NACT; mIndy; EIEE25
NCBI Protein Information
solute carrier family 13 member 5; Na(+)/citrate cotransporter; Na+-coupled citrate transporter protein; sodium-dependent dicarboxylate transporter
UniProt Protein Name
Solute carrier family 13 member 5
Protein Family
UniProt Gene Name
SLC13A5
UniProt Synonym Gene Names
NACT; NaCT
UniProt Entry Name
S13A5_HUMAN

NCBI Description

This gene encodes a protein belonging to the solute carrier family 13 group of proteins. This family member is a sodium-dependent citrate cotransporter that may regulate metabolic processes. Mutations in this gene cause early infantile epileptic encephalopathy 25. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]

Uniprot Description

SLC13A5: High-affinity sodium/citrate cotransporter that mediates citrate entry into cells. The transport process is electrogenic; it is the trivalent form of citrate rather than the divalent form that is recognized as a substrate. May facilitate the utilization of circulating citrate for the generation of metabolic energy and for the synthesis of fatty acids and cholesterol. Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, SLC family

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: basolateral plasma membrane; integral to membrane; plasma membrane

Molecular Function: citrate transmembrane transporter activity; succinate transmembrane transporter activity; sodium:dicarboxylate symporter activity

Biological Process: citrate transport; sodium ion transport; transmembrane transport

Disease: Epileptic Encephalopathy, Early Infantile, 25

Research Articles on SLC13A5

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Product Notes

The SLC13A5 slc13a5 (Catalog #AAA246619) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 2G4] (IgG1,k) to Human SLC13A5 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SLC13A5 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000) Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen. Researchers should empirically determine the suitability of the SLC13A5 slc13a5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC13A5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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