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Testing Data (Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine SLA 1:FITC)

Mouse SLA CLASS I Monoclonal Antibody

MOUSE ANTI PIG SLA CLASS I:FITC

Applications
Flow Cytometry, Functional Assay
Synonyms
SLA CLASS I; Monoclonal Antibody; MOUSE ANTI PIG SLA CLASS I:FITC; anti-SLA CLASS I antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
JM1E3
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Applicable Applications for anti-SLA CLASS I antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10 ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Pig
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine SLA 1:FITC)

Testing Data (Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine SLA 1:FITC)
Related Product Information for anti-SLA CLASS I antibody
Mouse anti Pig SLA Class I antibody, clone JM1E3 recognizes a monomorphic epitope expressed by porcine MHC class I molecules (SLA - 1). SLA - 1 is expressed by all nucleated porcine cells, but not on erythrocytes. This antibody has also been shown to cross-react with human MHC Class I, including HLA-E. (Galiani et al. 2002) The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. Mouse anti pig SLA class I, clone JM1E3 has been reported to block the interaction of MHC Class I antigens with inhibitory NK cell receptors (Galiani et al. 2002).

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Product Notes

The SLA CLASS I (Catalog #AAA215730) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SLA CLASS I can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10 ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the SLA CLASS I for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLA CLASS I, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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